Matsumoto K, Fujimoto M, Ito K, Tanaka H, Hirono I
Department of Pathology, School of Medicine, Fujita Health University, Aichi, Japan.
J Toxicol Sci. 1990 Feb;15(1):39-46. doi: 10.2131/jts.15.39.
Bilobol, isolated from ginkgo fruit pulp, has been noted to be a strong skin irritant like 12-O-tetradecanoylphorbol-13-acetate (TPA), a tumor-promoter in the skin. A comparative investigation of morphological changes induced by bilobol and TPA induced in the skin of CD-1 mice, and an assessment of the skin tumor promoting potential of bilobol were therefore performed. In experiment I, mice received a single application of 2.5, 50 or 1000 micrograms of bilobol, or 0.1 or 2.5 micrograms of TPA on the right ear. The 50 or 1000 micrograms bilobol and 2.5 micrograms TPA doses caused ear redness, epidermal thickening and inflammatory infiltration. The dose of 2.5 micrograms of TPA, which is usually used as tumor promoter in skin carcinogenesis, was equivalent to 50 micrograms of bilobol in irritant effect. Thus, 50 micrograms of bilobol was used for the promotion testing (experiment II) in CD-1 mice initiated with 100 micrograms of 7, 12-dimethylbenz[a]anthracene (DMBA). Treatment with either 10 or 50 micrograms bilobol twice a week for 30 weeks did not result in any tumor development, thus suggesting that bilobol is not a complete promoter of skin carcinogenesis, despite generation of inflammation.
从银杏果肉中分离出的白果醇，被发现是一种强烈的皮肤刺激物，类似于12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA），一种皮肤肿瘤促进剂。因此，对白果醇和TPA在CD - 1小鼠皮肤中诱导的形态学变化进行了比较研究，并评估了白果醇的皮肤肿瘤促进潜力。在实验I中，小鼠右耳单次涂抹2.5、50或1000微克白果醇，或0.1或2.5微克TPA。50或1000微克白果醇以及2.5微克TPA的剂量导致耳部发红、表皮增厚和炎症浸润。在皮肤致癌作用中通常用作肿瘤促进剂的2.5微克TPA剂量，在刺激效果上相当于50微克白果醇。因此，50微克白果醇被用于对用100微克7,12 - 二甲基苯并[a]蒽（DMBA）启动的CD - 1小鼠进行促进试验（实验II）。每周两次用10或50微克白果醇处理30周未导致任何肿瘤发生，这表明尽管会引发炎症，但白果醇并非皮肤致癌作用的完全促进剂。
