Aswad M I, John T, Barza M, Kenyon K, Baum J
Department of Ophthalmology, New England Medical Center, Boston, MA 02111.
Ophthalmology. 1990 Mar;97(3):296-302. doi: 10.1016/s0161-6420(90)32596-4.
The authors studied the adherence of Pseudomonas aeruginosa and Staphylococcus aureus to extended wear soft contact lenses (EWSCLs) with and without focal deposits using both a radiolabeling technique and electron microscopy. P. aeruginosa showed significant adherence to contact lenses in vitro. In contrast, S. aureus failed to show significant adherence to contact lenses in vitro (i.e., the radioactive uptake was not significantly above background). The extent of adherence of Pseudomonas was proportional to the number of focal deposits on the lenses. Results of electron microscopic examination showed the bacteria to be adherent primarily to large focal deposits (greater than or equal to 150 microns). There was no pseudomonal adherence to the small focal deposits (less than or equal to 50 microns) and little adherence to the areas in between the focal deposits. The authors hypothesize that worn lenses, especially those with large focal deposits, serve as a vehicle for the transport of P. aeruginosa to the cornea. This hypothesis could be a partial explanation for the high incidence of keratitis caused by P. aeruginosa in EWSCL patients.
作者使用放射性标记技术和电子显微镜，研究了铜绿假单胞菌和金黄色葡萄球菌对有或没有局灶性沉积物的长戴型软性隐形眼镜（EWSCL）的黏附情况。铜绿假单胞菌在体外对隐形眼镜表现出显著的黏附。相比之下，金黄色葡萄球菌在体外未表现出对隐形眼镜的显著黏附（即放射性摄取并未显著高于背景值）。铜绿假单胞菌的黏附程度与镜片上局灶性沉积物的数量成正比。电子显微镜检查结果显示，细菌主要黏附于大的局灶性沉积物（大于或等于150微米）。铜绿假单胞菌未黏附于小的局灶性沉积物（小于或等于50微米），且对局灶性沉积物之间的区域黏附较少。作者推测，佩戴过的镜片，尤其是那些有大的局灶性沉积物的镜片，充当了将铜绿假单胞菌转运至角膜的载体。这一推测可能是EWSCL患者中由铜绿假单胞菌引起的角膜炎高发病率的部分原因。
