在非宿主中获得可重复性会降低烟草轻型绿斑病毒在宿主中的沉默抑制活性。
Gaining replicability in a nonhost compromises the silencing suppression activity of Tobacco mild green mosaic virus in a host.
机构信息
Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
出版信息
J Virol. 2011 Feb;85(4):1893-5. doi: 10.1128/JVI.01741-10. Epub 2010 Nov 24.
Abstract
Natural isolates of Tobacco mild green mosaic virus (TMGMV) fail to infect tomato because the tomato tm-1 protein binds to the replication proteins of TMGMV and prevents RNA replication. Previously, we isolated a TMGMV mutant that overcomes tm-1-mediated resistance and multiplies in tomato plants. Here, we show that the causal mutations in the replication protein gene that abolish the interaction with tm-1 reduce its ability to suppress RNA silencing in host plant Nicotiana benthamiana. The results suggest that the multifunctionality of the replication proteins is an evolutionary constraint of tobamoviruses that restricts their host ranges.
摘要
烟草轻型绿斑病毒（TMGMV）的天然分离株无法感染番茄，因为番茄 tm-1 蛋白与 TMGMV 的复制蛋白结合，从而阻止 RNA 复制。此前，我们分离到一种克服 tm-1 介导抗性并在番茄植株中增殖的 TMGMV 突变体。在此，我们表明，复制蛋白基因中的功能丧失突变使该蛋白与 tm-1 互作的能力降低，从而降低其在宿主植物本氏烟中抑制 RNA 沉默的能力。结果表明，复制蛋白的多功能性是限制其宿主范围的烟草花叶病毒的进化约束。
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