Fahmy Inas Farouk, Taha Omnia, El-Ashry Abdel Nasser
Phytopathogen Vector Interaction Lab, Department of Microbiology, Agricultural Research Center (ARC), Agricultural Genetic Engineering Research Institute, Giza, Egypt.
Department of Plant Pathology, Faculty of Agriculture, Institute fuer Nutzpflanzenwissenschaften und Ressourcen Schutz, University of Bonn, Bonn, Germany.
Virusdisease. 2015 Jun;26(1-2):33-41. doi: 10.1007/s13337-014-0246-4. Epub 2015 Feb 14.
This study aims to identifying and characterizing some molecular properties of geminiviruses co-infection in squash field crop cultivated in Egypt. Squash crops observed to be heavily infected with several insect vectors, also severe chlorosis and stunting was observed. Electron microscopic analysis has revealed geminate capsid particles which indicate the infection of Geminiviruses, especially SqLCV which represent an economic problem to squash filed crop in Egypt. We have investigated possible mixed infections with different plant viruses associated with chlorotic stunt diseases and or other genus groups of geminiviruses. The main objective of this study is to investigate the recombination events, possible recombinants and variants among these genera in the same family differing in vector transmission. This is the first report of the molecular characterization, phylogenetic analysis and putative recombination events of the full length genome of the Chickpea Chlorotic Dwarf Mastrevirus in Egypt. And the first report of co-infection with another begomovirus infecting squash plants. A full length clone of both viruses were isolated and characterized at the molecular level. The complete nucleotide sequence of DNA-A was determined (2,572 bp) and submitted to the genbank under accession no. KF692356. The isolate from Egypt has about 97.8 % homology with the Chickpea chlorotic dwarf virus (CpCDV) isolate from Syria DNA-A isolate FR687959, a 83.2 % homology with the Sudan isolate AM933134 and a 82.7 % homology with Pakistan isolate FR687960. To best of our knowledge this is the first report of complete genome of CpCDV that infect squash plants in Egypt and worldwide.
本研究旨在鉴定和表征埃及种植的南瓜大田作物中双生病毒共感染的一些分子特性。观察到南瓜作物被多种昆虫载体严重感染，同时还出现了严重的黄化和发育迟缓现象。电子显微镜分析揭示了双联体衣壳颗粒，这表明双生病毒已感染，尤其是南瓜曲叶病毒（SqLCV），它给埃及的南瓜大田作物带来了经济问题。我们调查了与褪绿矮化病害相关的不同植物病毒以及双生病毒其他属组之间可能的混合感染情况。本研究的主要目的是调查这些在载体传播方面存在差异的同一科不同属之间的重组事件、可能的重组体和变体。这是埃及关于鹰嘴豆褪绿矮化玉米褪绿斑驳病毒全长基因组的分子特征、系统发育分析和推定重组事件的首次报道。也是关于另一种感染南瓜植株的菜豆金色花叶病毒属病毒共感染的首次报道。分离并在分子水平上表征了两种病毒的全长克隆。测定了DNA-A的完整核苷酸序列（2572 bp），并以登录号KF692356提交至基因库。来自埃及的分离株与来自叙利亚的鹰嘴豆褪绿矮化病毒（CpCDV）分离株DNA-A分离株FR687959具有约97.8%的同源性，与苏丹分离株AM933134具有83.2%的同源性，与巴基斯坦分离株FR687960具有82.7%的同源性。据我们所知，这是埃及乃至全球首次关于感染南瓜植株的CpCDV完整基因组的报道。
