Institut de Pharmacologie et de Biologie Structurale, IPBS-CNRS, 205 route de Narbonne, 31077 Toulouse, France.
Neurosci Lett. 2011 Jan 25;488(3):305-9. doi: 10.1016/j.neulet.2010.11.052. Epub 2010 Nov 24.
The present study investigates the modulatory effects of neuropeptide FF (NPFF) receptors on the mesolimbic dopaminergic pathway controlled by opioid receptors. A stable NPFF(2) receptor agonist, dNPA, was injected into the ventral tegmental area (VTA) and the release of dopamine and serotonin within the nucleus accumbens (NAc), induced by intraperitoneal injection of morphine, was monitored using the brain microdialysis, in non-constrained rat. dNPA decreased systemic morphine-induced elevation of dopamine and serotonin metabolites within the NAc. Furthermore, co-injected with morphine into the VTA, NPFF inhibited morphine-induced stereotypy 60-120min after the injection. This neurochemical and behavioural anti-opioid effect mediated by NPFF(2) receptors at the level of VTA suggests the involvement of NPFF in the rewarding effects of opiates on the mesolimbic dopamine system.
本研究探讨了神经肽 FF（NPFF）受体对阿片受体控制的中脑边缘多巴胺能通路的调节作用。采用脑微透析技术，在非束缚大鼠中，观察到稳定的 NPFF(2)受体激动剂 dNPA 注射到腹侧被盖区（VTA）后，腹腔注射吗啡诱导的伏隔核（NAc）内多巴胺和 5-羟色胺释放的变化。dNPA 降低了系统吗啡诱导的 NAc 内多巴胺和 5-羟色胺代谢物的升高。此外，当 NPFF 与吗啡一起注射到 VTA 时，NPFF 抑制了注射后 60-120 分钟吗啡诱导的刻板行为。这种由 VTA 水平的 NPFF(2)受体介导的神经化学和行为性抗阿片作用表明 NPFF 参与了阿片类药物对中脑边缘多巴胺系统的奖赏效应。
