DIPROVAL, Sezione di Allevamenti Zootecnici, University of Bologna, Via F.lli Rosselli 107, 42123 Reggio Emilia, Italy.
Genomics. 2011 Mar;97(3):158-65. doi: 10.1016/j.ygeno.2010.11.005. Epub 2010 Nov 24.
We carried out a cross species cattle-sheep array comparative genome hybridization experiment to identify copy number variations (CNVs) in the sheep genome analysing ewes of Italian dairy or dual-purpose breeds (Bagnolese, Comisana, Laticauda, Massese, Sarda, and Valle del Belice) using a tiling oligonucleotide array with ~385,000 probes designed on the bovine genome. We identified 135 CNV regions (CNVRs; 24 reported in more than one animal) covering ~10.5 Mb of the virtual sheep genome referred to the bovine genome (0.398%) with a mean and a median equal to 77.6 and 55.9 kb, respectively. A comparative analysis between the identified sheep CNVRs and those reported in cattle and goat genomes indicated that overlaps between sheep and both other species CNVRs are highly significant (P<0.0001), suggesting that several chromosome regions might contain recurrent interspecies CNVRs. Many sheep CNVRs include genes with important biological functions. Further studies are needed to evaluate their functional relevance.
我们进行了跨物种牛-羊基因组比较基因组杂交实验，使用设计在牛基因组上的约 385000 个探针的平铺寡核苷酸阵列，分析意大利乳用或兼用品种（Bagnolese、Comisana、Laticauda、Massese、Sarda 和 Valle del Belice）母羊的基因组，以鉴定绵羊基因组中的拷贝数变异（CNVs）。我们鉴定了 135 个 CNV 区域（CNVRs；在超过一种动物中报告了 24 个），涵盖了虚拟绵羊基因组中参考牛基因组的约 10.5 Mb（0.398%），平均值和中位数分别为 77.6 和 55.9 kb。鉴定的绵羊 CNVRs 与牛和山羊基因组中报告的 CNVRs 之间的比较分析表明，绵羊与其他两种物种的 CNVRs 之间的重叠高度显著（P<0.0001），这表明一些染色体区域可能包含反复出现的种间 CNVRs。许多绵羊 CNVRs 包含具有重要生物学功能的基因。需要进一步研究来评估它们的功能相关性。
