膜相关f1噬菌体外壳蛋白对大肠杆菌磷脂酰丝氨酸合成酶活性的影响。
Effect of membrane-associated f1 bacteriophage coat protein upon the activity of Escherichia coli phosphatidylserine synthetase.
作者信息
Chamberlain B K, Webster R E
出版信息
J Bacteriol. 1978 Sep;135(3):883-7. doi: 10.1128/jb.135.3.883-887.1978.
Abstract
The effects of insertion of the major coat protein of f1 bacteriophage into Escherichia coli membranes were investigated under conditions allowing in vivo analysis of phosphatidylserine synthesis. An E. coli strain possessing a temperature-sensitive phosphatidylserine decarboxylase was utilized under conditions in which the decarboxylase activity was reduced but nonlethal. The presence of the coat protein in the host membranes inhibits the activity of the phosphatidylserine synthetase and perhaps affects the activity of the phosphatidylserine decarboxylase.
摘要
在允许对磷脂酰丝氨酸合成进行体内分析的条件下，研究了将f1噬菌体的主要外壳蛋白插入大肠杆菌膜中的影响。利用一种具有温度敏感性磷脂酰丝氨酸脱羧酶的大肠杆菌菌株，在脱羧酶活性降低但非致死的条件下进行实验。宿主膜中存在外壳蛋白会抑制磷脂酰丝氨酸合成酶的活性，并且可能会影响磷脂酰丝氨酸脱羧酶的活性。
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