Meng F, Yokoyama H, Shirakashi S, Grabner D, Ogawa K, Ishimaru K, Sawada Y, Murata O
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Parasitol Int. 2011 Jan;60(1):90-6. doi: 10.1016/j.parint.2010.11.006. Epub 2010 Nov 26.
Kudoa prunusi n. sp. (Myxozoa; Multivalvulida) is described from the brain of Pacific bluefin tuna Thunnus orientalis cultured in Japan. Numerous white cysts, up to 0.5mm in size, were found on and in the brain. Spores having typically five spore valves and five polar capsules resembled a five-petal cherry blossom in apical view and were conical shape with a round bottom in side view. Average spore size was 9.63 (8.5-10.3) μm in width and 7.50 (6.7-8.6) μm in length. The spore dimensions of K. prunusi overlapped with those of Kudoa yasunagai ex Sillago ciliata having five to six spore valves, but they were clearly distinct in spore shape, 18S rDNA and 28S rDNA sequences (0.3% and 1.7% differences, respectively). Phylogenetic analysis of 18S rDNA revealed that K. prunusi grouped with the brain-infecting multivalvulid species, K. yasunagai, K. chaetodoni, K. lethrini and K. neurophila, rather than five-valved Kudoa spp. Combined with morphological, molecular and biological differences, K. prunusi was proven to be a new species.
普氏库道虫新种（粘孢子虫纲；多瓣虫目）是从日本养殖的太平洋蓝鳍金枪鱼（Thunnus orientalis）的脑中发现并描述的。在脑内和脑表面发现了大量白色囊肿，大小可达0.5毫米。孢子通常具有五个孢子瓣和五个极囊，从顶面观形似五瓣樱花，侧面观呈底部圆形的圆锥形。孢子平均宽度为9.63（8.5 - 10.3）微米，长度为7.50（6.7 - 8.6）微米。普氏库道虫的孢子尺寸与来自银鲈（Sillago ciliata）的安永库道虫（Kudoa yasunagai）（具有五到六个孢子瓣）的孢子尺寸有重叠，但在孢子形状、18S核糖体DNA和28S核糖体DNA序列上明显不同（分别有0.3%和1.7%的差异）。对18S核糖体DNA的系统发育分析表明，普氏库道虫与感染脑部的多瓣虫物种安永库道虫、鲹库道虫（K. chaetodoni）、裸颊鲷库道虫（K. lethrini）和嗜神经库道虫（K. neurophila）归为一类，而不是具有五个瓣的库道虫属物种。结合形态学、分子生物学和生物学差异，证实普氏库道虫是一个新物种。
