State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
J Hazard Mater. 2011 Feb 15;186(1):313-9. doi: 10.1016/j.jhazmat.2010.11.007. Epub 2010 Nov 9.
The shock load effect of heavy metals (Cu (II)) on the behavior of poly-phosphate-accumulating organisms (PAOs) was investigated with respect to the transformations of poly-P, intracellular polyhydroxyalkanoates (PHAs) and glycogen. The PAOs biomass was exposed to different concentrations of Cu (II) at various pH and biomass levels. The results showed that when the mixed liquor suspended solid (MLSS) concentration was 2500-4000 mg/L, the P removal was not adversely affected by spiking with 2 mg Cu(2+)/L; however, it deteriorated completely after a Cu (II) shock concentration of 4 mg/L. Nevertheless, the tolerance of PAOs biomass to Cu (II) shock could be enhanced by increasing the MLSS. Moreover, in the presence of 2 mg Cu(2+)/L, the P removal efficiency was highest at an initial pH of 6.2 and lowest at an initial pH of 6.9, indicating that the Cu inhibitory effect was reduced by increasing the pH to 7.6. The inhibition by Cu (II) was related to the transformation of intracellular storage compounds of PAOs. Specifically, poly-P degradation might be inhibited, which reduced the energy available for PHA production and eventually led to poor P removal.
重金属（Cu(II)）对聚磷酸盐积累菌（PAOs）行为的冲击负荷效应，以及聚磷酸盐（poly-P）、细胞内聚羟基烷酸酯（PHA）和糖原的转化。将 PAOs 生物量暴露于不同 pH 值和生物量水平的不同 Cu(II)浓度下。结果表明，当混合液悬浮固体（MLSS）浓度为 2500-4000mg/L 时，2mg/L 的 Cu(2+)不会对除磷产生不利影响；然而，当 Cu(II)冲击浓度为 4mg/L 时，除磷完全恶化。然而，通过增加 MLSS 可以增强 PAOs 生物量对 Cu(II)冲击的耐受性。此外，在 2mg/L Cu(2+)存在下，初始 pH 值为 6.2 时的除磷效率最高，初始 pH 值为 6.9 时的除磷效率最低，表明将 pH 值提高到 7.6 可以降低 Cu 的抑制作用。Cu(II)的抑制作用与 PAOs 细胞内储存化合物的转化有关。具体来说，聚磷酸盐的降解可能受到抑制，这减少了用于 PHA 生产的能量，最终导致除磷效果不佳。
