Yates N C, Moan J, Western A
Department of Biophysics, Norwegian Radium Hospital, Oslo.
J Photochem Photobiol B. 1990 Mar;4(4):379-90. doi: 10.1016/1011-1344(90)85017-q.
Metal naphthalocyanine complexes (MNCSs) absorb light in the near-IR spectral region (760 nm) where tissue penetration is optimal and they have been proposed as agents for photodynamic therapy (PDT). Sulphonated derivatives of tris-(2,3-naphthalocyanato) bis-chloroaluminium(III) and zinc(II) with various degrees of sulphonation were prepared. Cellular uptake, aggregation in cellular environments, cytotoxicity and photosensitizing properties were studied. Three of the four dyes studied were taken up by cells to a satisfactory degree and were not cytotoxic at the concentration used (10 micrograms ml-1). The least sulphonated sample of zinc naphthalocyanine produced some phototoxic effects (LD50 = 1.12 J cm-2). All the other samples of sulphonated naphthalocyanine were found to be aggregated inside the NHIK 3025 cells, preventing any significant PDT effect.
金属萘酞菁配合物（MNCSs）在近红外光谱区域（760纳米）吸收光，该区域组织穿透性最佳，它们已被提议作为光动力疗法（PDT）的试剂。制备了具有不同磺化程度的三（2,3 - 萘酞菁）双氯铝（III）和锌（II）的磺化衍生物。研究了细胞摄取、在细胞环境中的聚集、细胞毒性和光敏特性。所研究的四种染料中有三种被细胞摄取到令人满意的程度，并且在所使用的浓度（10微克/毫升）下没有细胞毒性。锌萘酞菁磺化程度最低的样品产生了一些光毒性效应（LD50 = 1.12焦/平方厘米）。发现所有其他磺化萘酞菁样品在NHIK 3025细胞内聚集，从而阻止了任何显著的光动力疗法效应。
