Almeida Camila B, Franco-Penteado Carla, Saad Sara T O, Costa Fernando F, Conran Nicola
Haematology and Haemotherapy Centre, State University of Campinas, Rua Carlos Chagas 480, Campinas, São Paulo, Brazil.
Hematology. 2010 Dec;15(6):422-9. doi: 10.1179/102453310X12719010991786.
Oxidative stress plays a significant role in sickle cell disease (SCD), contributing to haemolysis, vaso-occlusive processes and endothelial dysfunction. To study the effects that the serum of SCD individuals has on the oxidative state of blood cells, sera were pooled from control individuals, steady-state SCD patients and SCD patients on hydroxyurea therapy (SCDHU), and their effects on markers of oxidative stress and damage in neutrophils isolated from healthy individuals observed. Incubation of control neutrophils, but not platelets nor red blood cells, with SCD serum (10% v/v; 2 hours) significantly augmented their production of reactive oxygen species (ROS). Increased ROS production in SCD serum-incubated neutrophils was associated with increased superoxide anion generation, apoptosis and increased nicotinamide adenine dinucleotide phosphate oxidase subunit expression. Although serum from SCDHU individuals also induced ROS generation in neutrophils, its oxidative capacity appeared to be lower. Results suggest that factors in the serum of SCD individuals contribute to ROS generation and oxidative damage in leukocytes.
氧化应激在镰状细胞病（SCD）中起重要作用，导致溶血、血管阻塞过程和内皮功能障碍。为了研究SCD个体的血清对血细胞氧化状态的影响，收集了对照个体、稳态SCD患者和接受羟基脲治疗的SCD患者（SCDHU）的血清，并观察了它们对从健康个体分离的中性粒细胞中氧化应激和损伤标志物的影响。用SCD血清（10% v/v；2小时）孵育对照中性粒细胞，但不孵育血小板和红细胞，可显著增加其活性氧（ROS）的产生。SCD血清孵育的中性粒细胞中ROS产生增加与超氧阴离子生成增加、细胞凋亡和烟酰胺腺嘌呤二核苷酸磷酸氧化酶亚基表达增加有关。虽然SCDHU个体的血清也能诱导中性粒细胞产生ROS，但其氧化能力似乎较低。结果表明，SCD个体血清中的因素有助于白细胞中ROS的产生和氧化损伤。
