Zhou F, Zhu H, Wang L, Gao S
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, PLA, Beijing.
Wei Sheng Wu Xue Bao. 1990 Feb;30(1):7-15.
A procedure for obtaining gas chromatographic (GC) profiles of bacterial cellular monosaccharides was described. Some of the unknown component peaks in these profiles were identified. And, based on the complete linkage cluster analysis with the Euclidean distance coefficient, the interpretation of the resulting cellular monosaccharides of bacteria were performed by mini-computer. By means of this method, the discrimination of 5 species (24 strains) of aerobic endospore-forming bacteria. The results showed that there were defined differences between the profiles of cellular monosaccharides of B. anthracis and B. cereus. This procedure has provided a useful method for the classification and identification of microorganisms, for their physiological and biochemical studies, and for studies on their subcellular components.
描述了一种获取细菌细胞单糖气相色谱（GC）图谱的方法。这些图谱中的一些未知成分峰得到了鉴定。并且，基于使用欧几里得距离系数的完全连锁聚类分析，通过小型计算机对所得细菌细胞单糖进行了解释。通过这种方法，鉴别了5种（24株）需氧芽孢杆菌。结果表明，炭疽芽孢杆菌和蜡样芽孢杆菌的细胞单糖图谱存在明确差异。该方法为微生物的分类和鉴定、其生理生化研究以及亚细胞成分研究提供了一种有用的方法。
