Quantitation of nanoparticles in serum matrix by capillary electrophoresis.

作者信息

Chan King C, Veenstra Timothy D, Issaq Haleem J

机构信息

Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.

出版信息

Methods Mol Biol. 2011;697:145-53. doi: 10.1007/978-1-60327-198-1_15.

DOI:10.1007/978-1-60327-198-1_15

PMID:21116963

Abstract

Sensitive and fast analytical techniques are needed to determine the concentration of nanoparticles in biological samples (e.g., blood and tissues) for biodistribution and toxicity studies. This chapter describes a method for the use of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) for the quantitation of fullerene nanoparticles in human serum matrix. Data on the fullerene-based nanoparticle carboxyfullerene (C3 fullerene) in human serum is presented as an example.

摘要