Chan King C, Patri Anil K, Veenstra Timothy D, McNeil Scott E, Issaq Haleem J
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick Inc, NCI-Frederick, Frederick, MD 21702, USA.
Electrophoresis. 2007 May;28(10):1518-24. doi: 10.1002/elps.200600724.
With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C(60) derivatives were characterized by CZE using either a bare or dynamically coated fused-silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL.
随着将纳米颗粒用作药物递送载体和图像造影剂的兴趣日益增加，需要开发用于在复杂基质中检测和定量它们的分析方法，以促进对其生物分布的监测。在本研究中，我们开发了一种毛细管电泳（CE）方法，用于分析标准溶液和血清基质中的两种纳米颗粒：羧基富勒烯（C3）和树枝状富勒烯（DF1）。这些高度可溶的带电C60衍生物通过毛细管区带电泳（CZE）使用裸毛细管或动态涂层熔融石英毛细管进行表征。使用涂层毛细管时，两种纳米颗粒的分离度略低；然而，它们的迁移时间更快。虽然使用胶束电动毛细管色谱（MEKC）分离DF1纳米颗粒产生了更多可观察到的峰，但无论运行缓冲液中是否添加了十二烷基硫酸钠（SDS）胶束，C3的峰形基本不变。然后使用MEKC和/或CZE分析方法对加标的人血清样品中的C3和DF1纳米颗粒进行定量。纳米颗粒的定量在0 - 500μg/mL范围内呈线性，检测限为0.5至6μg/mL。
