Rodin D A, Abbot S D, Saade G, Clayton R N
Endocrinology Research Group, Clinical Research Centre, Harrow.
J Mol Endocrinol. 1990 Apr;4(2):159-67. doi: 10.1677/jme.0.0040159.
There are significant differences between rats and mice in the gonadal regulation of several aspects of gonadotroph function. To investigate whether these extend to the pretranslational regulation of FSH synthesis by gonadal steroids, we have measured FSH-beta mRNA levels following gonadectomy and sex-steroid replacement and have related these to serum and pituitary FSH as a reflection of overall hormone synthesis. In ovariectomized rats, FSH-beta mRNA levels increased by 8 h, decreased, and then rose progressively over the next 28 days. A similar pattern of response was observed in orchidectomized rats. In mice, there were progressive increases in FSH-beta mRNA levels in both males and females following gonadectomy, without evidence of the early peaks observed in rats. In both species, the change in FSH-beta mRNA levels after gonadectomy was greater in females than in males. These changes in FSH-beta mRNA following gonadectomy were paralleled by changes in the serum FSH concentration. In ovariectomized female rats and mice, pituitary FSH stores increased by 8 h and 3 days respectively, whereas in male rats, pituitary FSH content did not rise until 10 days after orchidectomy. The most striking species difference was the marked and prolonged reduction of pituitary FSH after orchidectomy of mice. Treatment of rats and mice from the time of ovariectomy, with a dose of oestradiol that prevents increases in serum LH, only partially attenuated the rises in FSH-beta mRNA and serum FSH and did not prevent the increase in pituitary FSH content. Treatment of intact or orchidectomized rats with testosterone suppressed FSH-beta mRNA levels to 50% below intact control values without affecting pituitary FSH content.(ABSTRACT TRUNCATED AT 250 WORDS)
在促性腺激素细胞功能的几个方面，大鼠和小鼠在性腺调节上存在显著差异。为了研究这些差异是否延伸至性腺类固醇对促卵泡激素（FSH）合成的转录前调节，我们测量了去势及性激素替代后FSH-β mRNA水平，并将其与血清和垂体FSH相关联，以反映整体激素合成情况。在去卵巢大鼠中，FSH-β mRNA水平在8小时时升高，随后下降，然后在接下来的28天中逐渐上升。在去势大鼠中观察到类似的反应模式。在小鼠中，去势后雄性和雌性的FSH-β mRNA水平均逐渐升高，没有大鼠中观察到的早期峰值迹象。在两个物种中，去势后雌性FSH-β mRNA水平的变化均大于雄性。去势后FSH-β mRNA的这些变化与血清FSH浓度的变化平行。在去卵巢的雌性大鼠和小鼠中，垂体FSH储存分别在8小时和3天时增加，而在雄性大鼠中，垂体FSH含量直到去势后10天才升高。最显著的物种差异是小鼠去势后垂体FSH显著且持续降低。从去卵巢时起用一定剂量的雌二醇治疗大鼠和小鼠，该剂量可防止血清促黄体生成素（LH）升高，仅部分减弱了FSH-β mRNA和血清FSH的升高，且未阻止垂体FSH含量的增加。用睾酮治疗完整或去势大鼠可将FSH-β mRNA水平抑制至低于完整对照值的50%，而不影响垂体FSH含量。（摘要截短至250字）
