Yeşiloğlu Yeşim, Şit Latifşah
Department of Chemistry, Faculty of Science, Trakya University, Edirne, Turkey.
Artif Cells Blood Substit Immobil Biotechnol. 2011 Aug;39(4):247-51. doi: 10.3109/10731199.2010.533125. Epub 2010 Nov 30.
Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.
来自皱落假丝酵母的脂肪酶（CRL）通过物理吸附固定在氧化铝上。在30分钟内固定化产率超过95%。还对游离酶和固定化酶的性质进行了研究和比较。通过固定化酶，最适pH从7变为8.5。游离酶和固定化酶的最大活性分别出现在35℃和40℃。
