游离及固定于氧化铝上的皱褶假丝酵母脂肪酶的生化特性：一项比较研究。
Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.
作者信息
Yeşiloğlu Yeşim, Şit Latifşah
机构信息
Department of Chemistry, Faculty of Science, Trakya University, Edirne, Turkey.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2011 Aug;39(4):247-51. doi: 10.3109/10731199.2010.533125. Epub 2010 Nov 30.
PMID:21117873
Abstract
Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.
摘要
来自皱落假丝酵母的脂肪酶（CRL）通过物理吸附固定在氧化铝上。在30分钟内固定化产率超过95%。还对游离酶和固定化酶的性质进行了研究和比较。通过固定化酶，最适pH从7变为8.5。游离酶和固定化酶的最大活性分别出现在35℃和40℃。
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