Mons N, Tison F, Geffard M
Immunology Lab., IBCN-CNRS, Université de Bordeaux II, France.
Neuroendocrinology. 1990 Apr;51(4):425-8. doi: 10.1159/000125369.
We demonstrate the presence of L-DOPA (L-3,4-dihydroxyphenylalanine)-labelled cell bodies and fibers in the rat preoptic and anterior hypothalamic areas, using a rabbit polyclonal antiserum against conjugated L-DOPA and a glutaraldehyde coupled immunohistochemical technique. In contrast, using a monoclonal anti conjugated dopamine (DA) antibody, no DA labelled neurons were detectable in the preoptic region, whereas a few weakly immunostained DA neurons began to appear in the ventral part of the medial preoptic area. These observations point to the hypothesis that these cells contain mainly L-DOPA neurons in the preoptic and anterior hypothalamic areas. Thus, we suggest that L-DOPA plays a more important role in the hypothalamic functions than has been hitherto assumed.
我们使用一种针对结合型L-多巴的兔多克隆抗血清和戊二醛偶联免疫组织化学技术，证明了大鼠视前区和下丘脑前部区域存在L-多巴（L-3,4-二羟基苯丙氨酸）标记的细胞体和纤维。相比之下，使用单克隆抗结合型多巴胺（DA）抗体，在视前区未检测到DA标记的神经元，而在视前内侧区腹侧开始出现一些弱免疫染色的DA神经元。这些观察结果支持了这样一种假说，即这些细胞在视前区和下丘脑前部区域主要包含L-多巴神经元。因此，我们认为L-多巴在下丘脑功能中发挥的作用比迄今所认为的更为重要。
