Bonsall R W, Zumpe D, Michael R P
Department of Psychiatry, Emory University School of Medicine, Atlanta, Ga.
Neuroendocrinology. 1990 Apr;51(4):474-80. doi: 10.1159/000125377.
Testosterone secreted by the testis of the macaque fetus is thought to influence certain aspects of the brain's subsequent development which may be responsible for the ontogeny of sexually dimorphic patterns of behavior. To compare the interactions between testosterone and the receptors for androgens and estrogens in brain cell nuclei in the two sexes, 7 intact female fetuses and 5 intact male fetuses were injected in utero at about 120 days of gestation with [3H]-testosterone (250 microCi i.v. or 500 microCi s.c.). One hour later, fetuses were delivered by cesarean section, and samples of brain and peripheral tissues were homogenized and separated into purified nuclear and supernatant fractions. Fractions were analyzed by high performance liquid chromatography to measure levels of [3H]-testosterone and its metabolites. Concentrations of radioactivity extracted from cell nuclei were significantly higher in the hypothalamus-preoptic area than in other brain areas (p less than 0.001); [3H]-estradiol represented 65.0 +/- 5.7% of this radioactivity and nuclear concentrations of this metabolite were 73% lower in males than in females (p less than 0.001). Nuclear concentrations of [3H]-testosterone in the pituitary gland (68.9 +/- 8.8% of extracted radioactivity) were 48% lower in males than in females (p less than 0.001). There was no evidence of a sex difference in the tissue uptake of radioactive steroids from blood, but in males, levels of endogenous plasma testosterone (599.8 +/- 208.2 ng/100 ml) were significantly higher than in females (37.7 +/- 28.5 ng/100 ml; p less than 0.01), and the specific activity of [3H]-testosterone in blood was consequently lower in males than in females.(ABSTRACT TRUNCATED AT 250 WORDS)
猕猴胎儿睾丸分泌的睾酮被认为会影响大脑后续发育的某些方面，这可能是两性行为性别差异模式个体发育的原因。为了比较睾酮与两性脑细胞核中雄激素和雌激素受体之间的相互作用，在妊娠约120天时，对7只完整的雌性胎儿和5只完整的雄性胎儿进行子宫内注射[3H] - 睾酮（静脉注射250微居里或皮下注射500微居里）。一小时后，通过剖宫产取出胎儿，将脑和外周组织样本匀浆并分离成纯化的细胞核和上清液部分。通过高效液相色谱分析各部分，以测量[3H] - 睾酮及其代谢物的水平。下丘脑 - 视前区从细胞核中提取的放射性浓度显著高于其他脑区（p < 0.001）；[3H] - 雌二醇占该放射性的65.0±5.7%，该代谢物的细胞核浓度在雄性中比雌性低73%（p < 0.001）。垂体中[3H] - 睾酮的细胞核浓度（占提取放射性的68.9±8.8%）在雄性中比雌性低48%（p < 0.001）。没有证据表明两性从血液中摄取放射性类固醇存在性别差异，但在雄性中，内源性血浆睾酮水平（599.8±208.2纳克/100毫升）显著高于雌性（37.7±28.5纳克/100毫升；p < 0.01），因此雄性血液中[3H] - 睾酮的比活性低于雌性。（摘要截断于250字）
