基于内在干扰的埃尔贡山蝙蝠病毒蚀斑试验。

A plaque assay for Mount Elgon bat virus based on intrinsic interference.

作者信息

Patel J R

出版信息

J Gen Virol. 1978 Sep;40(3):659-64. doi: 10.1099/0022-1317-40-3-659.

Abstract

A plaque count infectivity assay was developed in which chick cells infected with the Mount Elgon bat virus were completely resistant to superinfection with large doses of Sendai virus. Several variables markedly affected the assay sensitivity. The defined plaque assay was simple, highly reproducible and sensitive. It allowed determination of virus neutralizing antibody in a simple and reproducible test. Both actinomycin D and 5-iododeoxyuridine were without effect on plaque formation.

摘要

开发了一种蚀斑计数感染性测定法，其中感染了埃尔贡山蝙蝠病毒的鸡细胞对大剂量仙台病毒的再次感染完全具有抗性。几个变量显著影响测定的灵敏度。所定义的蚀斑测定法简单、高度可重复且灵敏。它能够在一个简单且可重复的试验中测定病毒中和抗体。放线菌素D和5-碘脱氧尿苷对蚀斑形成均无影响。