Kawamoto Y, Hanaichi T, Naito M, Miyama A
Department of Microbiology, Fujita-gakuen Health University School of Medicine, Aichi, Japan.
Experientia. 1990 May 15;46(5):495-8. doi: 10.1007/BF01954244.
Human myocardial cells from fresh autopsy material contained granules which possessed hemolytic activity against guinea pig and rabbit erythrocytes. The hemolytic granules, which had a density of 1.02 and a diameter of 200-300 nm, were recovered as a microsome fraction from subcellular homogenates of human myocardial cells by differential centrifugation in 300 mM sucrose containing 0.1 mM PMSF and 10 mM EDTA. The membrane lesions caused by the granules were ring-like structures with an internal diameter of about 10-17 nm, analogous to that caused by perforin- and complement-induced lysis. However, the requirement for divalent cation differed from that for perforin-induced lysis, since the microsome-mediated lysis occurred in the presence of EDTA.
来自新鲜尸检材料的人类心肌细胞含有对豚鼠和兔红细胞具有溶血活性的颗粒。溶血颗粒的密度为1.02，直径为200 - 300纳米，通过在含有0.1 mM苯甲基磺酰氟（PMSF）和10 mM乙二胺四乙酸（EDTA）的300 mM蔗糖中进行差速离心，从人类心肌细胞的亚细胞匀浆中作为微粒体部分回收。这些颗粒引起的膜损伤是内径约为10 - 17纳米的环状结构，类似于穿孔素和补体诱导的裂解所造成的损伤。然而，对二价阳离子的需求与穿孔素诱导的裂解不同，因为微粒体介导的裂解在EDTA存在的情况下发生。
