Podack E R, Dennert G
Nature. 1983;302(5907):442-5. doi: 10.1038/302442a0.
The formation of ultrastructural membrane lesions of varying size during cell mediated cytolysis effected by human peripheral blood leukocytes was recently reported by Dourmashkin et al. and Henkart et al. Using cloned mouse natural killer (NK) cells as effectors and YAC-1 cells or rabbit erythrocytes as targets, we now report two types of membrane lesions with inner diameters of 16 +/- 2 nm and approximately 5 nm, respectively. These lesions arise by membrane insertion of tubular complexes that may be assembled from subunits during the cytolytic reaction. The tubules are detected on target membranes by immune electron microscopy and appear to form transmembrane channels as seen in ultrathin sections. Both tubules are partially purified by membrane extraction with SDS and gel filtration in deoxycholate containing buffer. Based on the correlation of tubule assembly and cytolysis and on their detection on target membranes, we suggest that both types of tubules may be related to cytolysis.
多尔马什金等人和亨卡特等人最近报道了在人外周血白细胞介导的细胞溶解过程中形成的大小各异的超微结构膜损伤。以克隆的小鼠自然杀伤（NK）细胞作为效应细胞，YAC - 1细胞或兔红细胞作为靶细胞，我们现在报道了两种膜损伤，其内径分别为16±2纳米和约5纳米。这些损伤是由管状复合物插入膜中形成的，这些管状复合物可能在细胞溶解反应过程中由亚基组装而成。通过免疫电子显微镜在靶膜上检测到这些小管，并且在超薄切片中似乎形成了跨膜通道。两种小管都通过用SDS进行膜提取和在含有脱氧胆酸盐的缓冲液中进行凝胶过滤而部分纯化。基于小管组装与细胞溶解的相关性以及它们在靶膜上的检测结果，我们认为这两种类型的小管可能都与细胞溶解有关。
