Wood P R, Rothel J S, McWaters P G, Jones S L
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Vet Immunol Immunopathol. 1990 May;25(1):37-46. doi: 10.1016/0165-2427(90)90108-5.
Nine stable hybridoma cell lines were established which secreted specific monoclonal antibodies (MAbs) to bovine gamma-interferon (BoIFN-gamma). Specific binding of each of the MAbs to recombinant BoIFN-gamma (rBoIFN-gamma) was demonstrated in an indirect ELISA, whilst none of the MAbs bound to rBoIFN-alpha or rBoIFN-beta. In a Western blot the MAbs reacted with the 16 kDa and 32 kDa polypeptides present in rBoIFN-gamma preparations. Competitive ELISA's showed that four MAbs bound to one epitope on rBoIFN-gamma, and the other five MAbs bound to a separate epitope. Two MAbs, each recognising different epitopes, were shown to neutralise the anti-viral activity of natural BoIFN-gamma.
建立了9个稳定的杂交瘤细胞系，它们分泌针对牛γ-干扰素（BoIFN-γ）的特异性单克隆抗体（MAb）。在间接ELISA中证实了每种MAb与重组BoIFN-γ（rBoIFN-γ）的特异性结合，而没有一种MAb与rBoIFN-α或rBoIFN-β结合。在蛋白质印迹中，MAb与rBoIFN-γ制剂中存在的16 kDa和32 kDa多肽发生反应。竞争性ELISA表明，4种MAb结合到rBoIFN-γ上的一个表位，另外5种MAb结合到一个单独的表位。已证明两种识别不同表位的MAb可中和天然BoIFN-γ的抗病毒活性。
