Davies A G, Lawrence N R, Lynch S S
J Reprod Fertil. 1978 Jul;53(2):249-54. doi: 10.1530/jrf.0.0530249.
In-vivo testicular binding of highly purified pituitary FSH, labelled by a method which did not significantly affect biological potency, was hormone-specific, tissue-specific and dose-dependent. Hypophysectomy of mice was followed by a progressive increase in the amount of 125I-labelled FSH per unit weight of testis but not in the total amount of hormone taken up by the testis. Maximum binding occurred at 4 h in a membrane-containing fraction prepared by high-speed ultracentrifugation of testicular homogenate. This is considerably later than has been reported for testicular tissue incubated in vitro at 37 degrees C.
通过一种对生物活性影响不大的方法标记的高纯度垂体促卵泡激素（FSH）在体内的睾丸结合具有激素特异性、组织特异性和剂量依赖性。对小鼠进行垂体切除术后，每单位重量睾丸中125I标记的FSH量逐渐增加，但睾丸摄取的激素总量未增加。在通过睾丸匀浆高速超速离心制备的含膜部分中，4小时时出现最大结合。这比在37℃体外培养睾丸组织的报道时间要晚得多。
