Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas, France.
J Gen Virol. 2011 Mar;92(Pt 3):528-33. doi: 10.1099/vir.0.027417-0. Epub 2010 Dec 1.
We report here the first full-length sequence of the eight ssRNA genome segments of the infectious salmon anemia virus (ISAV, Glesvaer/2/90 isolate), a salmonid orthomyxovirus-like. Comparison of ISAV genome sequence with those of others orthomyxovirus reveals low identity values, and a remarkable feature is the extremely long 5' end UTR of ISAV segments, which all contain an additional conserved motif of unknown function. In addition to the genome nucleotide sequence determination, specific mAbs have been produced through mice immunization with sucrose-purified ISAV. Four mAbs directed against the haemagglutinin-esterase glycoprotein, the nucleoprotein and free or actin-associated forms of the matrix protein have been characterized by (i) indirect fluorescent antibody test; (ii) virus neutralization; (iii) radioimmunoprecipitation and (iv) Western blot assays. These mAbs will potentially be useful for the development of new diagnostic tests, and the nucleotide sequences will help to establish a reverse genetics system for ISAV.
我们在此报告传染性鲑鱼贫血病毒（ISAV，Glesvaer/2/90 分离株）的 8 个 ssRNA 基因组片段的全长序列，ISAV 是一种鲑鱼正粘病毒样病毒。ISAV 基因组序列与其他正粘病毒的比较显示出低的同一性值，一个显著的特征是 ISAV 片段的极长 5'UTR，其都包含一个未知功能的额外保守基序。除了基因组核苷酸序列测定外，还通过用蔗糖纯化的 ISAV 免疫小鼠产生了特异性 mAb。通过（i）间接荧光抗体试验；（ii）病毒中和；（iii）放射免疫沉淀和（iv）Western blot 分析，鉴定了针对血凝素-酯酶糖蛋白、核蛋白以及游离或肌动蛋白相关形式的基质蛋白的 4 种 mAb。这些 mAb 将有望用于开发新的诊断测试，核苷酸序列将有助于建立 ISAV 的反向遗传学系统。
