Department of Biotechnology, Thapar University, Patiala 147 004, India.
J Microbiol Biotechnol. 2010 Nov;20(11):1571-6. doi: 10.4014/jmb.1006.06032.
Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5 respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography (SEM) analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.
两种名为 CT2 和 CT5 的细菌菌株是使用富集培养技术从高碱性水泥样本中分离出来的。基于各种生理测试和 16S rRNA 序列分析，这些细菌被鉴定为芽孢杆菌属。CT2 和 CT5 的产脲酶活性分别为 575.87 U/ml 和 670.71 U/ml。CT2 和 CT5 堵塞的砂样中，方解石分别占总重量的 27.6%和 31%。扫描电子显微镜（SEM）分析表明这些分离物直接参与了方解石的沉淀。这是首次从水泥中分离和鉴定芽孢杆菌属的报道。基于这些细菌能够耐受水泥的极端环境，它们有可能用于修复各种建筑物或混凝土结构中的裂缝和裂隙。
