School of Pharmacy, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
Mol Pharm. 2011 Feb 7;8(1):117-25. doi: 10.1021/mp100137q. Epub 2010 Dec 20.
The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPARα. OCTN2 and PPARα expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical (IHC) analysis, Western blotting, and quantitative polymerase chain reaction (qPCR). Effects of hypoxia on the expression of OCTN2 and PPARα in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPARα protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPARα expression in human placental explants and in BeWo cells. WY14643, a PPARα agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPARα-mediated pathways.
本研究旨在探讨缺氧对胎盘 OCTN2 和 PPARα 表达的影响。通过免疫组织化学（IHC）分析、Western blot 和定量聚合酶链反应（qPCR）检测子痫前期存在或不存在时人胎盘中 OCTN2 和 PPARα 的表达。通过 Western blot 和 qPCR 分析检测缺氧对人胎盘绒毛外植体和人绒毛膜癌细胞 BeWo 中 OCTN2 和 PPARα 表达的影响。IHC、Western blot 和 qPCR 研究表明，与正常胎盘相比，子痫前期患者胎盘中合体滋养层的 OCTN2 和 PPARα 蛋白和 mRNA 水平较低。缺氧处理导致人胎盘绒毛外植体和 BeWo 细胞中 OCTN2 和 PPARα 的表达下降。PPARα 激动剂 WY14643 在缺氧条件下可引起 BeWo 细胞中 OCTN2 的表达增加。总之，在缺氧条件下，胎盘 OCTN2 通过 PPARα 介导的途径下调。
