Zhang Ya-Ting, Luo Zhao-Fan, Fang Jian-Pei, Guo Hai-Xia, Huang Ke, Li Chi-Kong
Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Oct;18(5):1235-9.
This study was purposed to detect the minimal residual disease (MRD) in childhood acute lymphoblastic leukemia (ALL) by using real time quantitative PCR (RQ-PCR) . The Ig and TCR gene rearrangements were amplified by using 18 primer sets in B-ALL, 8 primer sets in T-ALL; the ALL-MRD levels were quantified by using RQ-PCR with SYBR green dye staining and clone specific Ig/TCR gene rearrangements as molecular markers. The results indicated that there were 8 cases showing gene rearrangements in 9 B-ALL patients, marker detection rate for all samples was 88.8%, the MRD level on day 33 during induction treatment decreased significantly. It is concluded that Ig/TCR gene rearrangements can be used as a marker to detect MRD in childhood ALL; the technique of QR-PCR with SYBR green dye staining is reliable, relatively sensitive and easy performable method which can be used in routine detection for childhood ALL.
本研究旨在通过实时定量聚合酶链反应（RQ-PCR）检测儿童急性淋巴细胞白血病（ALL）中的微小残留病（MRD）。在B-ALL中使用18对引物扩增Ig和TCR基因重排，在T-ALL中使用8对引物；以SYBR绿染料染色的RQ-PCR和克隆特异性Ig/TCR基因重排作为分子标志物对ALL-MRD水平进行定量。结果显示，9例B-ALL患者中有8例出现基因重排，所有样本的标志物检测率为88.8%，诱导治疗第33天时MRD水平显著下降。结论：Ig/TCR基因重排可作为检测儿童ALL中MRD的标志物；SYBR绿染料染色的QR-PCR技术是一种可靠、相对敏感且易于操作的方法，可用于儿童ALL的常规检测。
