杏仁核内一氧化氮供体对组胺所致焦虑样效应的影响。
Influence of nitric oxide agents in the rat amygdala on anxiogenic-like effect induced by histamine.
机构信息
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Neurosci Lett. 2011 Feb 1;489(1):38-42. doi: 10.1016/j.neulet.2010.11.062. Epub 2010 Dec 2.
BACKGROUND/AIMS: Both histamine and nitric oxide (NO) may play a role in anxiety-like behavior. Within the brain, the amygdala is an important area involved in processing emotional responses such as anxiety. The aim of the present study was to assess whether the NO system in the basolateral amygdala (BLA) influences histamine-induced anxiety-like behavior in rats.
METHODS
Male Wistar rats weighing 200-220g were used. Bilateral cannulae were implanted in the BLA place for microinjections of drugs and the elevated plus maze apparatus has been used to test parameters (%OAT, %OAE, locomotor activity) of anxiety-like behavior.
RESULTS
Intra-BLA administration of histamine (2.5 and 5μg/rat) decreased %OAT [P<0.001]. Histamine (5μg/rat) also reduced %OAE [P<0.05] but not locomotor activity. The results obtained may indicate an anxiolytic response for histamine. Furthermore, bilateral intra-BLA microinjections of different doses of l-arginine (l-arg), an NO precursor (0.5 and 1μg/rat) increased %OAT [P<0.01], %OAE [P<0.01] and locomotor activity [P<0.001] while NG-nitro-l-arg methylester (l-NAME), a potent inhibitor of NO-synthase (NOS; 0.025, 0.05 and 0.1μg/rat) decreased %OAT [P<0.05] and locomotor activity [P<0.001] but not %OAE. The combination of l-arg (0.5μg/rat) with histamine increased %OAE [P<0.001] but had no effect on %OAT and locomotor activity. Finally, the combination of l-NAME (0.025μg/rat) with histamine decreased %OAT [P<0.001] and locomotor activity [P<0.05] but increased %OAE.
CONCLUSION
The results indicate a modulatory role for NO in BLA in the anxiogenic response of histamine in rats.
背景/目的：组胺和一氧化氮（NO）都可能在焦虑样行为中发挥作用。在大脑中，杏仁核是一个参与处理焦虑等情绪反应的重要区域。本研究的目的是评估外侧杏仁核（BLA）中的 NO 系统是否会影响大鼠中组胺引起的焦虑样行为。
方法
使用体重为 200-220g 的雄性 Wistar 大鼠。在 BLA 处植入双侧套管，用于药物微注射，高架十字迷宫装置用于测试焦虑样行为的参数（%OAT、%OAE、运动活动）。
结果
BLA 内给予组胺（2.5 和 5μg/大鼠）可降低 %OAT[P<0.001]。组胺（5μg/大鼠）还降低了 %OAE[P<0.05]，但不影响运动活动。结果表明组胺具有抗焦虑作用。此外，双侧 BLA 内微注射不同剂量的 l-精氨酸（l-arg），一种 NO 前体（0.5 和 1μg/大鼠），增加了 %OAT[P<0.01]、%OAE[P<0.01]和运动活动[P<0.001]，而 NG-硝基-l-精氨酸甲酯（l-NAME），一种强效的一氧化氮合酶（NOS）抑制剂（0.025、0.05 和 0.1μg/大鼠）降低了 %OAT[P<0.05]和运动活动[P<0.001]，但不影响 %OAE。l-arg（0.5μg/大鼠）与组胺合用增加了 %OAE[P<0.001]，但对 %OAT 和运动活动无影响。最后，l-NAME（0.025μg/大鼠）与组胺合用降低了 %OAT[P<0.001]和运动活动[P<0.05]，但增加了 %OAE。
结论
结果表明，NO 在 BLA 中对组胺在大鼠中的焦虑反应具有调节作用。
Zotero 插件