Kidney Disease Center, the First Affiliated Hospital, College of Medicine, Zhejiang University, China.
Transpl Immunol. 2011 Apr 15;24(3):172-80. doi: 10.1016/j.trim.2010.11.008. Epub 2010 Dec 1.
Effective non-invasive monitoring method to tell histopathology is a big challenge in renal transplantation.
We used 70-mer long oligonucleotide array with 449 immune related genes to determine gene expression profiles of peripheral blood mononuclear cells (PBMCs) under different immune status including stable renal function (TX), acute tubular necrosis (ATN), biopsy conformed acute rejection (AR), clinical rejection with pathology of borderline changes (BL), clinical rejection without biopsy proven/presumed rejection (PR) and renal dysfunction without rejection (NR).
Distinct molecular expression signatures in each group were found to correlate with histopathology. And we concluded that B cell chemokine CXCL13 and mast cell may play a role in renal allograft rejection through significant difference analysis and functional pathway analysis.
It provides a potential non-invasive method for monitoring renal allograft function and immune status of renal transplant recipients.
寻找一种有效的非侵入性监测方法来判断组织病理学变化是肾移植领域的一大挑战。
我们使用带有 449 个免疫相关基因的 70 -mer 长寡核苷酸阵列，来确定外周血单个核细胞（PBMCs）在不同免疫状态下的基因表达谱，这些免疫状态包括肾功能稳定（TX）、急性肾小管坏死（ATN）、经活检证实的急性排斥反应（AR）、临床排斥反应伴有边界变化的病理学（BL）、临床排斥反应但无活检证实/推测排斥反应（PR）和无排斥反应的肾功能障碍（NR）。
我们发现每组的分子表达特征与组织病理学相关。通过差异分析和功能途径分析，我们得出结论，B 细胞趋化因子 CXCL13 和肥大细胞可能通过显著差异分析和功能途径分析在肾移植排斥反应中发挥作用。
该研究为监测肾移植受者的肾移植功能和免疫状态提供了一种潜在的非侵入性方法。
