Pruul H, Goodwin C S, McDonald P J, Lewis G, Pankhurst D
Department of Microbiology, Flinders Medical Centre, Bedford Park, South Australia.
J Med Microbiol. 1990 Jun;32(2):93-100. doi: 10.1099/00222615-32-2-93.
Cell-surface hydrophobicity of Helicobacter (formerly Campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. The hydrophobicity of H. pylori greatly exceeded that of Campylobacter fetus subsp. fetus, C. jejuni and Bacillus subtilis. A partition coefficient (PC) of hydrophobicity in the two-phase system was determined for H. pylori. PC was dependent on pH and the PC value was increased by greater than 20-fold at pH 2.5. Lithium cations increased PC, indicating a net negative surface charge. The presence of urea prevented the relative loss of hydrophobicity at raised pH. Exposure of H. pylori to proteolytic enzymes reduced the ability of the bacteria to adhere to human polymorphonuclear neutrophils (PMN). These findings suggest that H. pylori possesses protein-associated hydrophobic factors that are responsible for the non-opsonic adherence to PMN cell membranes.
通过水相两相分配和疏水相互作用色谱法检测幽门螺杆菌（原弯曲菌属）的细胞表面疏水性。幽门螺杆菌的疏水性大大超过胎儿弯曲菌胎儿亚种、空肠弯曲菌和枯草芽孢杆菌。测定了幽门螺杆菌在两相系统中的疏水性分配系数（PC）。PC取决于pH值，在pH 2.5时PC值增加超过20倍。锂阳离子增加PC，表明表面净负电荷。尿素的存在可防止在升高的pH值下疏水性的相对丧失。幽门螺杆菌暴露于蛋白水解酶会降低细菌与人多形核中性粒细胞（PMN）粘附的能力。这些发现表明，幽门螺杆菌具有与蛋白质相关的疏水因子，这些因子负责对PMN细胞膜的非调理素粘附。
