Department of Civil and Environmental Engineering, University of Missouri, E2509 Lafferre Hall, Columbia, MO 65211, USA.
Water Res. 2011 Jan;45(3):1157-64. doi: 10.1016/j.watres.2010.11.002. Epub 2010 Nov 20.
Nitrogen removal mainly relies on sequential nitrification and denitrification in wastewater treatment. Microbial fuel cells (MFCs) are innovative wastewater treatment techniques for pollution control and energy generation. In this study, bench-scale wastewater treatment systems using membrane-aerated MFC (MAMFC) and diffuser-aerated MFC (DAMFC) techniques were constructed for simultaneous removal of carbonaceous and nitrogenous pollutants and electricity production from wastewater. During 210 days of continuous flow operation, when the dissolved oxygen (DO) in the cathodic compartment was kept at 2 mg/L, both reactors demonstrated high COD removal (>99%) and high ammonia removal (>99%) but low nitrogen removal (<20%). When a lower DO (0.5 mg/L) was maintained after day 121, both the MFC-based reactors still had excellent COD removal (>97%). However, the nitrogen removal of MAMFC (52%) was 2-fold higher than that of DAMFC (24%), indicating an enhanced performance of denitrification after DO reduction in the cathodic compartment of the MAMFC. Meanwhile, terminal restriction fragment length polymorphism (T-RFLP) analysis of ammonia-oxidizing bacteria (AOB) population in the MAMFC indicated the diversity of AOB with equally important Nitrosospira and Nitrosomonas species present in the cathodic biofilm after DO reduction. The average voltage output in the MAMFC was significantly higher than that in DAMFC under both DO conditions. The results suggest that MAMFC systems have the potential for wastewater treatment with improved nitrogen removal and electricity production.
氮的去除主要依赖于废水处理中的序批式硝化反硝化。微生物燃料电池（MFC）是一种用于污染控制和能源产生的创新废水处理技术。在这项研究中，使用膜曝气 MFC（MAMFC）和扩散曝气 MFC（DAMFC）技术构建了台式废水处理系统，用于从废水中同时去除碳质和氮质污染物并发电。在 210 天的连续流动操作中，当阴极室中的溶解氧（DO）保持在 2 mg/L 时，两个反应器均表现出高 COD 去除率（>99%）和高氨氮去除率（>99%），但氮去除率低（<20%）。当在第 121 天后将 DO 维持在较低水平（0.5 mg/L）时，基于 MFC 的两个反应器仍具有出色的 COD 去除率（>97%）。然而，MAMFC 的氮去除率（52%）是 DAMFC（24%）的两倍，这表明在降低阴极室 DO 后，MAMFC 的反硝化性能得到了增强。同时，MAMFC 中氨氧化菌（AOB）种群的末端限制性片段长度多态性（T-RFLP）分析表明，在降低 DO 后，阴极生物膜中的 AOB 多样性增加，其中 Nitrosospira 和 Nitrosomonas 等重要物种均存在。在两种 DO 条件下，MAMFC 的平均电压输出均明显高于 DAMFC。结果表明，MAMFC 系统具有改善氮去除和发电的废水处理潜力。
