运输温度对公羊附睪尾精子质量的影响。
Effect of transportation temperature on the quality of cauda epididymal spermatozoa of ram.
机构信息
Division of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry, S. K. University of Agricultural Sciences and Technology (K), Shuhama, Srinagar, Kashmir, India.
出版信息
Anim Reprod Sci. 2011 Jan;123(1-2):54-9. doi: 10.1016/j.anireprosci.2010.10.012. Epub 2010 Nov 4.
The objective of this study was to develop a protocol for ram epididymal sperm preservation that could be applied to wild ruminants for collection and preservation of spermatozoa from dead or hunted animals. Ram testicles collected from abattoirs were used to study the effect of two transportation temperatures viz. ambient temperature (AT) and refrigeration temperature (RT) on the cauda epididymal sperm quality at recovery and during preservation up to 72h at 4°C. For AT the testicles were transported in normal saline in a container (17.9-21.5°C) where as for RT the testicles were transported in an ice-chest (4.9-6°C). The results of the current study revealed that intact acrosome was significantly higher (P<0.01) and other quality parameters like sperm motility, live sperm count, sperm concentration and major sperm abnormalities were also higher (P>0.05) for RT than AT. The mean percent sperm motility for RT and AT was 81.67% and 78.33%, respectively. The corresponding figures were 92.08% and 90.46% for mean live sperm, 98.33% and 90.50% for intact acrosome, 0.50% and 0.33% for major sperm defects. The percent minor abnormality was 79.50% for RT and 77.67% for AT. The most prevalent minor defect was distal cytoplasmic droplet (70-80%). The mean sperm motility for RT and AT at 0h was 82.50% and 75.00%, respectively and the corresponding values at 72h of preservation were 60.00% and 45.83%. The mean live sperm at 0h for RT and AT were 92.92% and 88.92%, respectively and the corresponding figures at 72h were 81.50% and 73.17%. The mean intact acrosome at 0h for RT and AT was 98.58% and 90.58%, respectively and at 72h the corresponding values were 91.66% and 82.25%. The sperm motility, live sperm count and intact acrosome decreased significantly (P<0.05) from 0h to 72h of preservation for both transportation temperatures. The sperm motility, live sperm count and intact acrosome also varied significantly between the transportation temperatures. The major sperm abnormality for both RT and AT at each hour of preservation up to 72h was less than 0.5%. The study concluded that epididymides or testicles should be transported to the laboratory at RT (4.9-6°C) either in an ice-chest or portable refrigerator for their processing, evaluation and storage.
本研究的目的是制定一种公羊附睪精子保存方案，该方案可应用于野生反刍动物，用于收集和保存死亡或被猎杀动物的精子。本研究使用从屠宰场收集的公羊睾丸，研究了两种运输温度（环境温度（AT）和冷藏温度（RT））对回收时和在 4°C 下保存至 72 小时时的尾部附睪精子质量的影响。对于 AT，睾丸在容器中用生理盐水运输（17.9-21.5°C），而对于 RT，睾丸在冷藏箱中运输（4.9-6°C）。目前研究的结果表明，完整顶体的比例明显更高（P<0.01），其他质量参数，如精子活力、活精子计数、精子浓度和主要精子异常也更高（P>0.05）对于 RT 比 AT。RT 和 AT 的平均精子活力分别为 81.67%和 78.33%。对应的数字分别为 92.08%和 90.46%用于平均活精子，98.33%和 90.50%用于完整顶体，0.50%和 0.33%用于主要精子缺陷。次要异常的百分比为 RT 的 79.50%和 AT 的 77.67%。最常见的次要缺陷是远端细胞质小滴（70-80%）。RT 和 AT 的精子活力在 0 小时分别为 82.50%和 75.00%，在 72 小时的保存期分别为 60.00%和 45.83%。RT 和 AT 的活精子在 0 小时分别为 92.92%和 88.92%，在 72 小时的相应数值分别为 81.50%和 73.17%。RT 和 AT 的完整顶体在 0 小时的比例分别为 98.58%和 90.58%，在 72 小时的相应数值分别为 91.66%和 82.25%。精子活力、活精子计数和完整顶体从 0 小时到 72 小时的保存显著降低（P<0.05），适用于两种运输温度。精子活力、活精子计数和完整顶体在运输温度之间也有显著差异。在每个小时的保存期内，RT 和 AT 的主要精子异常均小于 0.5%。本研究得出的结论是，应将附睪或睾丸在 RT（4.9-6°C）下运输到实验室，无论是在冷藏箱中还是便携式冰箱中，以进行处理、评估和储存。
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