Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, SP 18610-970, Brazil.
Anim Reprod Sci. 2011 Sep;127(3-4):197-201. doi: 10.1016/j.anireprosci.2011.08.002. Epub 2011 Aug 16.
The cryopreservation of epididymal sperm is important to preserve genetic material from valuable deceased males. This study evaluated the viability of sperm samples from eight stallions under three conditions: (1) collected using an artificial vagina (EJ-0h), (2) recovered from the epididymal cauda immediately after orchiectomy (EP-0h), and (3) recovered from the epididymal cauda after 24h of storage at 5°C (EP-24h). To obtain EJ-0h sperm, two ejaculates were collected from each stallion. After 1 week, the stallions were submitted to bilateral orchiectomy, and one of the removed epididymides was flushed to obtain EP-0h sperm. The contralateral epididymis was stored at 5°C for 24h before being flushed to obtain EP-24h sperm. The sperm samples were analyzed at three different times: immediately after sperm recovery, after dilution in the freezing extender, and post-thawing. A fertility trial was performed using 39 estrous cycles. After ovulation induction with 1mg of deslorelin acetate (i.m.), mares were inseminated with 800×10(6) sperm. The total number of sperm recovered was 7.8±4.7×10(9) for EJ-0h sperm, 12.9±9.2×10(9) for EP-0h sperm and 12.0±8.0×10(9) for EP-24h sperm. The sperm motility, evaluated by total motility, progressive motility and the percentage of rapid cells, was similar among the samples before and after freezing (P>0.05). However, the plasma membrane integrity was different between EJ-0h and EP-0h pre-freezing and between EJ-0h and EP-24h post-thawing (P<0.05). The conception rates were similar between groups inseminated with sperm recovered from the epididymal cauda immediately after orchiectomy (EP-0h), after 24h of storage at 5°C of the epididymal cauda (EP-24h) and with ejaculated sperm (EJ-0h) (P>0.05). In conclusion, the viability and fertility of cauda epididymal sperm are similar to those of ejaculated sperm.
附睾精子的冷冻保存对于保存有价值的死亡雄性的遗传物质非常重要。本研究评估了 8 匹种马在三种条件下的精子样本的活力：(1) 使用人工阴道收集(EJ-0h)，(2) 睾丸切除后立即从附睾尾部回收(EP-0h)，和(3) 在 5°C 下储存 24 小时后从附睾尾部回收(EP-24h)。为了获得 EJ-0h 精子，从每匹种马采集两个精液样本。1 周后，种马接受双侧睾丸切除术，并从切除的一个附睾中冲洗以获得 EP-0h 精子。对侧附睾在 5°C 下储存 24 小时，然后冲洗以获得 EP-24h 精子。精子样本在三个不同时间点进行分析：精子回收后立即、在冷冻稀释剂中稀释后和解冻后。使用 39 个发情周期进行了一次受精试验。用 1mg 去势激素释放激素类似物(肌肉注射)诱导排卵后，用 800×10(6)个精子给母马授精。回收的精子总数分别为 EJ-0h 精子 7.8±4.7×10(9)个、EP-0h 精子 12.9±9.2×10(9)个和 EP-24h 精子 12.0±8.0×10(9)个。精子活力，通过总活力、前向运动精子和快速细胞的百分比进行评估，在冷冻前后的样本之间相似(P>0.05)。然而，冷冻前 EJ-0h 和 EP-0h 之间以及解冻后 EJ-0h 和 EP-24h 之间的质膜完整性不同(P<0.05)。立即从睾丸切除后回收的附睾尾部精子(EP-0h)、5°C 下储存 24 小时的附睾尾部精子(EP-24h)和射出的精子(EJ-0h)的授精组的妊娠率相似(P>0.05)。总之，附睾尾部精子的活力和生育力与射出的精子相似。
