ST11，中国产 KPC 肺炎克雷伯菌的优势克隆。

ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China.

机构信息

Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

出版信息

J Antimicrob Chemother. 2011 Feb;66(2):307-12. doi: 10.1093/jac/dkq431. Epub 2010 Dec 3.

DOI:10.1093/jac/dkq431

PMID:21131324

Abstract

OBJECTIVES

Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae has spread rapidly in China. In this study, we aimed to investigate the molecular epidemiology of KPC-producing K. pneumoniae isolates in China.

METHODS

Ninety-five carbapenem-resistant K. pneumoniae isolates from 13 hospitals in nine cities covering five provinces in China were analysed. Antibiotic susceptibility was determined by the Etest. Multilocus sequence typing (MLST) and PFGE were used for epidemiological analysis. The genetic structure around bla(KPC) and the encoding genes of extended-spectrum β-lactamases and plasmid-mediated AmpC enzymes were detected by PCR and sequencing. Plasmids were analysed by transformation, restriction and Southern blot.

RESULTS

All isolates harboured the bla(KPC-2) gene. Seven sequence types (STs) were obtained. The dominant clone was ST11 (61/95), which was identified in isolates from Zhejiang province (four hospitals in Hangzhou and one hospital in Ningbo), Jiangsu province (one hospital in Nanjing) and Anhui province (one hospital in Hefei). Isolates with ST11 showed >80% similarity in PFGE patterns. Plasmids from 14 selected transformants, their original isolates representing different STs and/or regions, had a diversity of HindIII restriction maps.

CONCLUSIONS

The dominant clone of KPC-producing K. pneumoniae in China is ST11, which is closely related to ST258, which has been reported worldwide.

摘要

目的

产碳青霉烯酶肺炎克雷伯菌（KPC）在中国迅速传播。本研究旨在探讨中国产 KPC 肺炎克雷伯菌分离株的分子流行病学。

方法

分析了来自中国五个省九个城市的 13 家医院的 95 株耐碳青霉烯类肺炎克雷伯菌。药敏试验采用 Etest 法。多位点序列分型（MLST）和 PFGE 用于流行病学分析。PCR 和测序检测 bla(KPC)周围的遗传结构和广谱β-内酰胺酶及质粒介导 AmpC 酶的编码基因。通过转化、限制和 Southern 印迹分析质粒。

结果

所有分离株均携带 bla(KPC-2)基因。得到 7 种序列型（ST）。优势克隆为 ST11（95/95），在浙江（杭州 4 家医院和宁波 1 家医院）、江苏（南京 1 家医院）和安徽（合肥 1 家医院）的分离株中均有发现。PFGE 图谱相似性>80%的菌株均为 ST11 型。来自 14 个选定转化子的质粒，其原始分离株代表不同的 ST 和/或地区，具有多样性的 HindIII 限制图谱。