de Castro Pimentel Figueiredo Bárbara, de Castro Patrícia Alves, Dinamarco Taísa Magnani, Goldman Maria Helena S, Goldman Gustavo Henrique
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil.
Eukaryot Cell. 2011 Feb;10(2):276-83. doi: 10.1128/EC.00224-10. Epub 2010 Dec 3.
Upon apoptosis induction, translocation of mammalian mitochondrial endonuclease G (EndoG) to the nucleus coincides with large-scale DNA fragmentation. Here, we describe for the first time a homologue of EndoG in filamentous fungi by investigating if the Aspergillus nidulans homologue of the EndoG gene, named nucA(EndoG), is being activated during farnesol-induced cell death. Our results suggest that NucA is not involved in cell death, but it plays a role in the DNA-damaging response in A. nidulans.
在诱导细胞凋亡时，哺乳动物线粒体核酸内切酶G（EndoG）向细胞核的转位与大规模DNA片段化同时发生。在此，我们通过研究法尼醇诱导的细胞死亡过程中构巢曲霉EndoG基因的同源物（命名为nucA(EndoG)）是否被激活，首次描述了丝状真菌中EndoG的同源物。我们的结果表明，NucA不参与细胞死亡，但它在构巢曲霉的DNA损伤反应中发挥作用。
