采用表型、基因组和转录组分析相结合的综合方法评估乳脂乳球菌亚种的多样性。
Assessment of the diversity of dairy Lactococcus lactis subsp. lactis isolates by an integrated approach combining phenotypic, genomic, and transcriptomic analyses.
机构信息
Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France.
出版信息
Appl Environ Microbiol. 2011 Feb;77(3):739-48. doi: 10.1128/AEM.01657-10. Epub 2010 Dec 3.
Abstract
The intrasubspecies diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in an ultrafiltration cheese model. The six strains were isolated from various sources, but all exhibited a dairy phenotype (growth in ultrafiltration cheese model and high acidification rate). The six strains exhibited similar behaviors in terms of growth during cheese ripening, while different acidification capabilities were detected. Even if all strains displayed large genomic similarities, sharing a large core genome of almost 2,000 genes, the expression of this core genome directly in the cheese matrix revealed major strain-specific differences that potentially could account for the observed different acidification capabilities. This work demonstrated that significant transcriptomic polymorphisms exist even among Lactococcus lactis subsp. lactis strains with the same dairy origin.
摘要
在超滤奶酪模型中，从基因组水平、表型和转录组谱方面研究了 6 株乳球菌乳亚种的种内多样性。这 6 株菌均分离自不同来源，但均表现出乳脂状（在超滤奶酪模型中生长和高酸化率）。在奶酪成熟过程中，这 6 株菌的生长行为相似，而酸化能力则不同。尽管所有菌株的基因组相似度较大，共享了近 2000 个基因的大型核心基因组，但该核心基因组在奶酪基质中的直接表达显示出主要的菌株特异性差异，这可能解释了观察到的不同酸化能力。这项工作表明，即使是具有相同乳源的乳球菌乳亚种菌株之间，也存在显著的转录组多态性。
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