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利用聚合酶链反应对正常和免疫缺陷B淋巴细胞的II类人类白细胞抗原基因进行转录分析。

Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction.

作者信息

Bull M, van Hoef A, Gorski J

机构信息

Immunogenetics Research Section, Blood Center of Southeastern, Wisconsin, Milwaukee 53233.

出版信息

Mol Cell Biol. 1990 Jul;10(7):3792-6. doi: 10.1128/mcb.10.7.3792-3796.1990.

DOI:10.1128/mcb.10.7.3792-3796.1990
PMID:2113177
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360836/
Abstract

The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.

摘要

通过对cDNA进行聚合酶链反应扩增，从II类先天性免疫缺陷B细胞中测定了所有人类白细胞抗原II类基因座的RNA转录水平。在能检测到0.01%正常水平的条件下未观察到mRNA。前体mRNA可从正常B细胞中扩增出来，但不能从免疫缺陷B细胞中扩增出来，这表明存在转录缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/a869ee8b4e7d/molcellb00043-0519-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/583551b38f98/molcellb00043-0517-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/b12599c7e16a/molcellb00043-0518-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/db70017c30d9/molcellb00043-0518-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/56bdc1abc0cb/molcellb00043-0518-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/552582dc0415/molcellb00043-0518-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/a869ee8b4e7d/molcellb00043-0519-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/583551b38f98/molcellb00043-0517-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/b12599c7e16a/molcellb00043-0518-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/db70017c30d9/molcellb00043-0518-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/56bdc1abc0cb/molcellb00043-0518-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/552582dc0415/molcellb00043-0518-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c4/360836/a869ee8b4e7d/molcellb00043-0519-a.jpg

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本文引用的文献

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