利用聚合酶链反应对正常和免疫缺陷B淋巴细胞的II类人类白细胞抗原基因进行转录分析。
Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction.
作者信息
Bull M, van Hoef A, Gorski J
机构信息
Immunogenetics Research Section, Blood Center of Southeastern, Wisconsin, Milwaukee 53233.
出版信息
Mol Cell Biol. 1990 Jul;10(7):3792-6. doi: 10.1128/mcb.10.7.3792-3796.1990.
Abstract
The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.
摘要
通过对cDNA进行聚合酶链反应扩增，从II类先天性免疫缺陷B细胞中测定了所有人类白细胞抗原II类基因座的RNA转录水平。在能检测到0.01%正常水平的条件下未观察到mRNA。前体mRNA可从正常B细胞中扩增出来，但不能从免疫缺陷B细胞中扩增出来，这表明存在转录缺陷。
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