Department of Obstetrics and Gynecology, E. Wolfson Medical Center, Holon, Israel.
Int J Gynecol Pathol. 2011 Jan;30(1):41-5. doi: 10.1097/PGP.0b013e3181f29c6e.
The aim of the study was to assess the expression of cyclooxygenases (COX)-2 in nonepithelial ovarian malignancies.COX-2 immunohistochemical staining was performed on newly prepared deparaffinized slides from formalin-fixed, paraffin-embedded archival tissue blocks of unselected nonepithelial ovarian malignancies diagnosed between January 1993 and October 2009 after reconfirmation of the diagnosis. Staining was assessed according to intensity of staining and the proportion of stained cells. Staining of more than 10% of the cells was considered positive.During the study period, 26 histologically proven nonepithelial ovarian malignancies were diagnosed. Of them, 16 were granulosa cell tumors and 10 were germ cell tumors (4 dysgerminomas and 6 immature teratomas). Five (31.2%) granulosa cell tumors had positive immunohistochemical COX-2 staining. Positive staining was observed only in 1 immature teratoma and in none of the dysgerminomas.Our data seem to indicate that COX-2 expression by immunohistochemical methods is not frequent in nonepithelial ovarian malignancies.
本研究旨在评估环氧合酶（COX）-2 在非上皮性卵巢恶性肿瘤中的表达。COX-2 的免疫组织化学染色在新制备的脱蜡切片上进行，这些切片来自 1993 年 1 月至 2009 年 10 月间诊断的未经选择的非上皮性卵巢恶性肿瘤的福尔马林固定、石蜡包埋存档组织块，这些肿瘤在重新确认诊断后被收录。染色根据染色强度和染色细胞的比例进行评估。超过 10%的细胞染色被认为是阳性的。在研究期间，诊断出 26 例经组织学证实的非上皮性卵巢恶性肿瘤。其中，16 例为颗粒细胞瘤，10 例为生殖细胞肿瘤（4 例为无性细胞瘤和 6 例为不成熟畸胎瘤）。5 例（31.2%）颗粒细胞瘤 COX-2 免疫组织化学染色阳性。仅在 1 例不成熟畸胎瘤中观察到阳性染色，而在无性细胞瘤中均未观察到阳性染色。我们的数据似乎表明，COX-2 的表达在非上皮性卵巢恶性肿瘤中通过免疫组织化学方法并不常见。
