蛋白酪氨酸激酶 7 在 Wnt/β-连环蛋白经典信号通路中具有保守作用。
Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling.
机构信息
Inserm, U891, Centre de Recherche en Cancérologie de Marseille, 27 Boulevard Lei Roure, Marseille F-13009.
出版信息
EMBO Rep. 2011 Jan;12(1):43-9. doi: 10.1038/embor.2010.185. Epub 2010 Dec 3.
Abstract
The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakened β-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require β-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.
摘要
受体酪氨酸激酶 7（PTK7）最近被证明在小鼠和青蛙胚胎发育过程中参与非经典的 Wnt/平面细胞极性信号转导。在这项研究中，我们报告说，PTK7 在酵母双杂交测定和哺乳动物细胞中与β-连环蛋白相互作用。PTK7 缺陷细胞在 Wnt3a 刺激下表现出较弱的β-连环蛋白/T 细胞因子转录活性。此外，Xenopus PTK7 是 Spemann 组织者形成所必需的，也是 Siamois 启动子激活所必需的，这些事件需要β-连环蛋白的转录活性。通过上位性测定，我们证明了 PTK7 在糖原合成激酶 3 的上游发挥作用。总之，我们的数据揭示了 PTK7 在 Wnt 经典信号通路中的一个新的和保守的作用。
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