Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand Excellence Center for Tissue Engineering and Stem Cells, Chiang Mai 50200, Thailand.
In Vitro Cell Dev Biol Anim. 2011 Mar;47(3):183-7. doi: 10.1007/s11626-010-9375-2. Epub 2010 Dec 4.
Alpinia galanga has been used as alternative medicine for anti-rheumatic activities. However, the precise action of the extract on arthritic diseases is not yet fully understood. In this study, we investigated the effects of A. galanga extracts on the expression of genes involved in catabolic activities in an interleukin-1β (IL-1β)-induced human synovial fibroblast as an inflammatory model. Confluent primary human synovial fibroblasts were treated for 24 h with A. galanga hexane extracts in the presence of recombinant human IL-1β. MMPs in the culture medium were monitored by gelatin zymography. Total RNA was isolated from the cell lysate and analyzed via semi-quantitative RT-PCR. After treatment with A. galanga extracts, MMP-2 activity in the culture medium was significantly reduced. In addition, MMP-1, MMP-3, MMP-13, and Cox-2 expression were downregulated. These data suggest that the decrease of gene expression and production of MMPs in synovial fibroblasts against inflammatory stimuli could be due to the effects of the A. galanga extracts. Therefore, A. galanga extracts might be a promising therapeutic agent for arthritis.
高良姜被用作治疗风湿性疾病的替代药物。然而，其提取物对关节炎疾病的确切作用尚未完全了解。在这项研究中，我们研究了 A. galanga 提取物对白细胞介素-1β（IL-1β）诱导的人滑膜成纤维细胞炎症模型中参与分解代谢活性的基因表达的影响。将与人滑膜成纤维细胞共培养的细胞用 A. galanga 己烷提取物处理 24 小时，同时加入重组人 IL-1β。通过明胶酶谱法监测培养基中的 MMPs。从细胞裂解物中分离总 RNA，并通过半定量 RT-PCR 进行分析。用 A. galanga 提取物处理后，培养基中 MMP-2 的活性明显降低。此外，MMP-1、MMP-3、MMP-13 和 Cox-2 的表达也下调。这些数据表明，滑膜成纤维细胞对炎症刺激的基因表达和 MMPs 的产生减少可能是由于 A. galanga 提取物的作用。因此，A. galanga 提取物可能是一种有前途的关节炎治疗药物。
