Department of Pharmacology,Faculty of Pharmacy,University of Seville,Profesor García González, 2, 41012 Seville,Spain.
Br J Nutr. 2019 Jan;121(1):55-62. doi: 10.1017/S0007114518002829. Epub 2018 Oct 26.
The polyphenolic extract (PE) from extra virgin olive oil (EVOO) has been shown to possess important anti-inflammatory and joint protective properties in murine models of rheumatoid arthritis (RA). This study was designed to evaluate the effects of PE on IL-1β-activated human synovial fibroblasts SW982 cell line. PE from EVOO treatment inhibited IL-1β-induced matrix metalloproteases (P<0·001), TNF-α and IL-6 production (P<0·001). Similarly, IL-1β-induced cyclo-oxygenase-2 and microsomal PGE synthase-1 up-regulations were down-regulated by PE (P<0·001). Moreover, IL-1β-induced mitogen-activated protein kinase (MAPK) phosphorylation and NF-κB activation were ameliorated by PE (P<0·001). These results suggest that PE from EVOO reduces the production of proinflammatory mediators in human synovial fibroblasts; particularly, these protective effects could be related to the inhibition of MAPK and NF-κB signalling pathways. Taken together, PE from EVOO probably could provide an attractive complement in management of diseases associated with over-activation of synovial fibroblasts, such as RA.
特级初榨橄榄油的多酚提取物 (PE) 已被证明在类风湿关节炎 (RA) 的小鼠模型中具有重要的抗炎和关节保护特性。本研究旨在评估 PE 对 IL-1β 激活的人滑膜成纤维细胞 SW982 细胞系的影响。EVOO 处理的 PE 抑制了 IL-1β 诱导的基质金属蛋白酶（P<0·001）、TNF-α 和 IL-6 的产生（P<0·001）。同样，PE 下调了 IL-1β 诱导的环加氧酶-2 和微粒体 PGE 合酶-1 的上调（P<0·001）。此外，PE 改善了 IL-1β 诱导的丝裂原活化蛋白激酶 (MAPK) 磷酸化和 NF-κB 激活（P<0·001）。这些结果表明，EVOO 的 PE 减少了人滑膜成纤维细胞中促炎介质的产生；特别是，这些保护作用可能与抑制 MAPK 和 NF-κB 信号通路有关。综上所述，EVOO 的 PE 可能为管理与滑膜成纤维细胞过度激活相关的疾病（如 RA）提供一种有吸引力的补充治疗方法。
