Chen Daiqin, Chen Chuanhuizi, Du Dan
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China.
J Nanosci Nanotechnol. 2010 Sep;10(9):5662-6. doi: 10.1166/jnn.2010.2477.
Acetylcholinesterase (AChE) activity may be useful biomarker for detecting of organophosphate pesticides (OP). Thus a sensitive biosensor for quantitative determination of OP based on AChE biomonitoring was developed. Multi-walled carbon nanotube (MWCNT)/polyaniline (PANI) composite film was prepared by electrochemical polymerization. The immobilized AChE catalyzed the hydrolysis of acetylthiocholine chloride to produce thiocholine, which engendered an irreversible oxidation peak. The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity.
乙酰胆碱酯酶（AChE）活性可能是检测有机磷农药（OP）的有用生物标志物。因此，开发了一种基于AChE生物监测的用于定量测定OP的灵敏生物传感器。通过电化学聚合制备了多壁碳纳米管（MWCNT）/聚苯胺（PANI）复合膜。固定化的AChE催化氯化乙酰硫代胆碱水解产生硫代胆碱，硫代胆碱产生不可逆的氧化峰。通过测量硫代胆碱的氧化电流监测酶活性，并进一步检测OP。所开发的传感器为农药分析和酶活性测定提供了一种新的有前景的工具。
