Targońska K, Kucharczyk D
Department of Lake & River Fisheries, Warmia & Mazury University, Olsztyn, Poland.
Reprod Domest Anim. 2011 Aug;46(4):651-5. doi: 10.1111/j.1439-0531.2010.01723.x. Epub 2010 Dec 6.
Artificial reproduction of fish is one of the main goals of aquaculture production. The aim of this study is to optimize the method of goldfish reproduction under controlled conditions by comparing the effectiveness of carp pituitary homogenate (CPH), Ovopel and human chorionic gonadotropin (hCG), administered as a one-off dose and inducing two spawns in the same fish within a short time period. Goldfish spawners were stimulated with hCG, CPH and Ovopel, and the results were compared to the fish from the control group, comprised of unstimulated fish. In another experiment, spawn were induced twice within an interval of 21 days with the same group of fish. The best results in the first experiment in terms of the percentage of ovulating females and survival to the eyed-egg stage were achieved after administering hCG (100% and 88.7%, respectively). However, the highest fecundity was observed in fish stimulated with Ovopel (89,960 eggs/kg). It was shown in the second experiment that female goldfish produce higher weight of eggs during the first spawning, but the number of eggs/BW ratio was higher during the next reproduction process. Survival, both that of embryos to the eyed-egg stage and that of spawners, is higher during the first reproduction act.
鱼类的人工繁殖是水产养殖生产的主要目标之一。本研究的目的是通过比较鲤鱼脑垂体匀浆（CPH）、Ovopel和人绒毛膜促性腺激素（hCG）一次性给药并在短时间内诱导同一条鱼产两次卵的效果，来优化在可控条件下金鱼的繁殖方法。用hCG、CPH和Ovopel刺激金鱼产卵亲鱼，并将结果与由未受刺激的鱼组成的对照组的鱼进行比较。在另一项实验中，用同一组鱼在21天的间隔内诱导产卵两次。在第一次实验中，就排卵雌鱼的百分比和到眼点卵阶段的存活率而言，注射hCG后取得了最佳结果（分别为100%和88.7%）。然而，用Ovopel刺激的鱼观察到最高的繁殖力（89,960枚卵/千克）。第二次实验表明，雌性金鱼在第一次产卵时产出的卵重量更高，但在下一次繁殖过程中卵数/体重比更高。在第一次繁殖过程中，胚胎到眼点卵阶段的存活率以及产卵亲鱼的存活率都更高。
