Department of Ecology and Evolutionary Biology, Rice University, Houston, TX 77005, USA.
Mol Ecol. 2010 Dec;19(24):5328-31. doi: 10.1111/j.1365-294X.2010.04889.x.
The inevitable has happened: genomic technologies have been added to our noninvasive genetic sampling repertoire. In this issue of Molecular Ecology, Perry et al. (2010) demonstrate how DNA extraction from chimpanzee faeces, followed by a series of steps to enrich for target loci, can be coupled with next-generation sequencing. These authors collected sequence and single-nucleotide polymorphism (SNP) data at more than 600 genomic loci (chromosome 21 and the X) and the complete mitochondrial DNA. By design, each locus was 'deep sequenced' to enable SNP identification. To demonstrate the reliability of their data, the work included samples from six captive chimps, which allowed for a comparison between presumably genuine SNPs obtained from blood and potentially flawed SNPs deduced from faeces. Thus, with this method, anyone with the resources, skills and ambition to do genome sequencing of wild, elusive, or protected mammals can enjoy all of the benefits of noninvasive sampling.
基因组技术已经被添加到我们的非侵入性遗传采样工具中。在本期《分子生态学》中，Perry 等人（2010）展示了如何从黑猩猩粪便中提取 DNA，然后通过一系列步骤富集目标基因座，再结合下一代测序技术。这些作者在 600 多个基因组基因座（21 号染色体和 X 染色体）和完整的线粒体 DNA 上收集了序列和单核苷酸多态性（SNP）数据。通过设计，每个基因座都进行了“深度测序”，以识别 SNP。为了证明他们数据的可靠性，这项工作包括来自六只圈养黑猩猩的样本，这使得他们能够比较从血液中获得的可能是真实 SNP 和从粪便中推断出的可能有缺陷的 SNP。因此，通过这种方法，任何有资源、技能和雄心壮志的人都可以对野生的、难以捉摸的或受保护的哺乳动物进行基因组测序，同时享受非侵入性采样的所有好处。
