Laboratory of Immunovirology, Biomedicine Institute of Seville, Service of Infectious Diseases, Virgen del Rocío University Hospital, Seville, Spain.
Antiviral Res. 2011 Feb;89(2):182-5. doi: 10.1016/j.antiviral.2010.11.015. Epub 2010 Dec 4.
Enhanced sensitivity Trofile (ES-Trofile) is the most frequently used technique to assay HIV tropism. A clinical approach to predict CCR5-antagonists efficacy, based on the virological response to a short-term maraviroc exposure (Maraviroc Clinical Test, MCT), has been recently reported. We compared the results of ES-Trofile with MCT in 47 HIV-infected patients, and a global discordance around 15% was observed between the phenotypic method and the clinical approach. Discordance results were mainly found in patients with an ES-Trofile reported as dual/mixed. These provocative results might have important clinical implications and should be considered in order to accurately prescribe treatment with CCR5 antagonists.
增强敏感性分析（ES-Trofile）是目前最常用于检测 HIV 嗜性的技术。最近有报道称，基于短期马拉维若治疗的病毒学反应，采用临床方法预测 CCR5 拮抗剂的疗效。我们比较了 47 例 HIV 感染患者的 ES-Trofile 和 MCT 结果，表型方法和临床方法之间存在约 15%的整体差异。不一致的结果主要出现在报告为双重/混合的 ES-Trofile 患者中。这些有争议的结果可能具有重要的临床意义，应该加以考虑，以便准确地开具 CCR5 拮抗剂治疗。
