Jilin Provincial Academy of Chinese Medicine Sciences, Changchun 130021, China.
J Ethnopharmacol. 2011 Mar 8;134(1):136-40. doi: 10.1016/j.jep.2010.11.071. Epub 2010 Dec 4.
Thladiantha dubia (Cucurbitaceae) has been used in Chinese manchu nationality folk medicine for treatment of various diseases, such as scrofula, carbuncle, lactation deficiency, etc. Its effects include softening hard masses, heat-clearing and detoxifying, reducing swelling, eliminating carbuncle, and promoting lactation. It has a history of use in the treatment of breast disorders and as an agent to increase lactation. The aim of the present study was to evaluate the anti-hyperplasia of mammary (HMG) effect of T. dubia root ethanol extract (TD-E) in estrogen combined with progestogen induced HMG rats.
Forty virgin female Wistar rats were randomly divided into blank control, HMG model, Rupixiao Tablet (RPXT, positive control), and TD-E groups, 10 in each. Injection of estrogen and progestogen were given to establish rat models of HMG, RPXT and TD-E were given at the same time. Changes of nipple heights were measured; uterus and ovary index were calculated; sex hormone levels (LH, E(2), and P) in serum were estimated. Pathologic changes of MG in rats were also observed under light microscope.
TD-E could decrease the increased nipple height, reduce the numbers of MG lobules and relieve the pathologic changes of HMG in rats. It could also decrease E(2) and LH levels in serum compared with the control group, nipple height, uterus index, and the levels of E(2) and LH in the HMG model group increased significantly, the level of P decreased significantly. Compared with the HMG model group, nipple height, uterus index, and the levels of E(2) and LH in the TD-E group decreased, and no obvious effect on P content. The histopathological analysis suggested that TD-E obviously alleviated the degree of HMG. Those results suggest TD-E has protective and therapeutic effects on HMG rats induced by estrogen and progestogen.
三叶鬼针草（葫芦科）在满族民间医学中用于治疗各种疾病，如瘰疬、痈疽、乳汁不足等。其作用包括软坚散结、清热解毒、消肿排脓、催乳。它有治疗乳腺疾病和增加泌乳的应用历史。本研究旨在评价三叶鬼针草根乙醇提取物（TD-E）对雌孕激素联合诱导的乳腺增生（HMG）大鼠的抗增生作用。
40 只处女 Wistar 大鼠随机分为空白对照组、HMG 模型组、乳癖消片（RPXT，阳性对照组）和 TD-E 组，每组 10 只。注射雌激素和孕激素建立 HMG 大鼠模型，同时给予 RPXT 和 TD-E。测量乳头高度的变化；计算子宫和卵巢指数；估计血清中性激素水平（LH、E2 和 P）。还在光镜下观察大鼠 MG 的病理变化。
TD-E 可降低增高的乳头高度，减少乳腺小叶数量，缓解 HMG 大鼠的病理变化。与对照组相比，它还可以降低血清中 E2 和 LH 水平，与 HMG 模型组相比，乳头高度、子宫指数以及 E2 和 LH 水平显著升高，P 水平显著降低。与 HMG 模型组相比，TD-E 组乳头高度、子宫指数以及 E2 和 LH 水平降低，对 P 含量无明显影响。组织病理学分析表明，TD-E 明显减轻了 HMG 的程度。这些结果表明 TD-E 对雌孕激素诱导的 HMG 大鼠具有保护和治疗作用。
