Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Mol Phylogenet Evol. 2011 Feb;58(2):232-43. doi: 10.1016/j.ympev.2010.11.023. Epub 2010 Dec 4.
Species identifications have been historically difficult in the Choristoneura fumiferana group, an important insect pest complex. We examined the utility of simple sequence repeats (SSRs, also referred to as microsatellites) and mitochondrial DNA (mtDNA) for delimiting and identifying eight currently recognized species sampled across North America. Four of these species formed discrete clusters using SSRs, while only two species were delimited with mtDNA. There was evidence for hybridization or incomplete lineage sorting between several species pairs. An integrative approach, using both phenotypic traits and molecular markers, allowed for the discrimination of more biologically relevant species units than did the use of molecular markers alone. As species are currently identified using putatively adaptive phenotypic traits, the differences observed between recognized species and neutral SSRs or mtDNA suggests that these species (or evolutionary significant units) have diverged via natural selection in spite of some gene flow.
物种鉴定在 Choristoneura fumiferana 组中一直具有挑战性，该组是一个重要的昆虫害虫复合体。我们研究了简单重复序列（SSR，也称为微卫星）和线粒体 DNA（mtDNA）在划定和鉴定跨越北美的八个目前公认的物种中的效用。使用 SSR，其中四个物种形成离散的聚类，而只有两个物种使用 mtDNA 划定。在几个物种对之间存在杂交或不完全谱系分选的证据。整合使用表型特征和分子标记的方法允许区分比仅使用分子标记更具生物学意义的物种单位。由于目前使用假定的适应性表型特征来鉴定物种，因此在公认的物种与中性 SSR 或 mtDNA 之间观察到的差异表明，尽管存在一些基因流，但这些物种（或具有进化意义的单位）已经通过自然选择而分化。
