食物过敏草药配方 2 可通过抑制肥大细胞和嗜碱性粒细胞来预防花生过敏反应。
Food allergy herbal formula 2 protection against peanut anaphylactic reaction is via inhibition of mast cells and basophils.
机构信息
Division of Allergy and Immunology, Department of Pediatrics, Mount Sinai School of Medicine, New York, NY 10029-6574, USA.
出版信息
J Allergy Clin Immunol. 2010 Dec;126(6):1208-17.e3. doi: 10.1016/j.jaci.2010.09.013.
BACKGROUND
Mast cells and basophils are key effector cells of IgE-mediated anaphylactic reactions. The Chinese herbal formula, food allergy herbal formula 2 (FAHF-2), protects against peanut anaphylaxis in mice. However, the mechanisms underlying this effect are not fully elucidated.
OBJECTIVE
To investigate whether FAHF-2 inhibits mast cell/basophil numbers and IgE-mediated activation.
METHODS
Mice with peanut allergy (PNA mice) were treated with FAHF-2 intragastrically for 7 weeks and challenged intragastrically with peanut 1 day and 4 weeks posttreatment. Peripheral blood basophil numbers and peritoneal mast cell numbers and FcεRI expression were determined. Direct effects of FAHF-2 on the murine mast cell line MC/9, and effects of 4 fractions and 3 compounds isolated from FAHF-2 on rat basophilic leukemia cells (RBL-2H3) and human skin mast cells degranulation and on the IgE-mediated spleen tyrosine kinase signaling pathway, were determined.
RESULTS
Although all sham-treated PNA mice developed anaphylaxis, FAHF-2-treated PNA mice were protected against anaphylaxis after peanut challenge at 1 day and 4 weeks posttherapy. Reduction of peripheral blood basophils began after 1 week of treatment and continued for at least 4 weeks posttherapy. The number and FcεRI expression of peritoneal mast cells were also significantly decreased 4 weeks posttherapy. FAHF-2-treated MC/9 cells showed significantly reduced IgE-induced FcεRI expression, FcεRI γ mRNA subunit expression, proliferation, and histamine release on challenge. Fraction 2 from FAHF-2 inhibited RBL-2H3 cell and human mast cell degranulation. Three compounds from fraction 2-berberine, palmatine, and jatrorrhizine-inhibited RBL-2H3 cell degranulation via suppressing spleen tyrosine kinase phosphorylation.
CONCLUSION
Food allergy herbal formula 2 reduction of basophils and mast cell numbers as well as suppression of IgE-mediated mast cell activation may contribute to FAHF-2's persistent protection against peanut anaphylaxis.
背景
肥大细胞和嗜碱性粒细胞是 IgE 介导的过敏反应的关键效应细胞。中草药配方食物过敏草药配方 2（FAHF-2）可保护小鼠免受花生过敏。然而，其作用机制尚未完全阐明。
目的
研究 FAHF-2 是否抑制肥大细胞/嗜碱性粒细胞数量和 IgE 介导的激活。
方法
用 FAHF-2 灌胃治疗花生过敏（PNA）小鼠 7 周，在治疗后 1 天和 4 周时经胃内挑战花生。检测外周血嗜碱性粒细胞数和腹腔肥大细胞数及 FcεRI 表达。测定 FAHF-2 对鼠肥大细胞系 MC/9 的直接作用，以及从 FAHF-2 中分离的 4 个馏分和 3 个化合物对大鼠嗜碱性白血病细胞（RBL-2H3）和人皮肤肥大细胞脱颗粒以及 IgE 介导的脾酪氨酸激酶信号通路的作用。
结果
尽管所有假处理 PNA 小鼠均发生过敏反应，但在治疗后 1 天和 4 周时经胃内挑战花生后，FAHF-2 治疗的 PNA 小鼠均受到保护，免受过敏反应。治疗后 1 周开始减少外周血嗜碱性粒细胞，并持续至少 4 周。治疗后 4 周，腹腔肥大细胞的数量和 FcεRI 表达也明显减少。FAHF-2 处理的 MC/9 细胞在 IgE 诱导的 FcεRI 表达、FcεRIγmRNA 亚基表达、增殖和组胺释放方面显示出明显减少。FAHF-2 的馏分 2 抑制 RBL-2H3 细胞和人肥大细胞脱颗粒。馏分 2 中的 3 种化合物——小檗碱、巴马汀和药根碱——通过抑制脾酪氨酸激酶磷酸化来抑制 RBL-2H3 细胞脱颗粒。
结论
FAHF-2 降低嗜碱性粒细胞和肥大细胞数量以及抑制 IgE 介导的肥大细胞激活可能有助于其对花生过敏的持续保护。
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