Guéry J C, Druet P
INSERM U 28, Hôpital Broussais, Paris, France.
Eur J Immunol. 1990 May;20(5):1027-31. doi: 10.1002/eji.1830200512.
Anti-idiotypic (Id) antibodies have been suggested to play a role in the self regulation process observed in Brown-Norway rats developing mercury-induced autoimmunity. However, the presence of such antibodies has not yet been directly demonstrated. For that purpose, spleen cells from a mercury-injected rat were fused and the resulting hybridomas tested for their anti-Id activity against monoclonal anti-glomerular basement membrane (GBM) antibodies produced in this model. A monoclonal antibody (mAb) was obtained that specifically reacted in an enzyme-linked immunosorbent assay with an anti-GBM mAb and to a much lesser extent with another one produced in the same fusion. In Western blot experiments this autoanti-Id mAb reacted under reducing conditions with the kappa L chains but not with the H chains of the two anti-GBM mAb. It did not react with the kappa L chains of eight other rat mAb. This mAb is therefore an autoanti-Id mAb that recognizes a V kappa-associated Id expressed on two anti-GBM mAb. These results demonstrate that anti-GBM antibodies and their corresponding autoanti-Id antibodies are simultaneously produced during this disease. Whether or not these autoanti-Id antibodies have a regulatory and/or a pathogenic role in this disease remains to be established.
抗独特型（Id）抗体被认为在发生汞诱导自身免疫的棕色挪威大鼠中观察到的自我调节过程中发挥作用。然而，此类抗体的存在尚未得到直接证实。为此，将注射汞的大鼠的脾细胞进行融合，并检测所得杂交瘤针对该模型中产生的单克隆抗肾小球基底膜（GBM）抗体的抗Id活性。获得了一种单克隆抗体（mAb），其在酶联免疫吸附测定中与抗GBM mAb特异性反应，与同一融合中产生的另一种抗体的反应程度则小得多。在蛋白质印迹实验中，这种自身抗Id mAb在还原条件下与两种抗GBM mAb的κ轻链反应，但不与重链反应。它不与其他八种大鼠mAb的κ轻链反应。因此，这种mAb是一种自身抗Id mAb，可识别在两种抗GBM mAb上表达的与Vκ相关的Id。这些结果表明，在这种疾病期间同时产生了抗GBM抗体及其相应的自身抗Id抗体。这些自身抗Id抗体在这种疾病中是否具有调节和/或致病作用仍有待确定。
