Braun P E, Horvath E, Yong V W, Bernier L
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
J Neurosci Res. 1990 May;26(1):16-23. doi: 10.1002/jnr.490260103.
Myelin membranes purified from mouse and rat brain are associated with alpha subunits of four signal transducing guanosine triphosphate (GTP)-binding proteins: Go, Gi, Gs, and ras. Four low-molecular-weight (Mr) GTP-binding proteins are also present, as demonstrated by the binding of GTP to proteins immobilized in nitrocellulose. This latter group is more prominent at early stages of myelination and remains associated with isolated myelin membranes despite repetitive cycles of purification. At least one nonmyelin subcellular membrane fraction possesses the same proteins. The total membrane fraction of cultured oligodendrocytes is associated with both groups of GTP-binding proteins. None of the well-known myelin proteins bound GTP by the procedure described.
从小鼠和大鼠脑中纯化的髓磷脂膜与四种信号转导鸟苷三磷酸（GTP）结合蛋白的α亚基相关：Go、Gi、Gs和ras。通过GTP与固定在硝酸纤维素上的蛋白质结合证明，还存在四种低分子量（Mr）GTP结合蛋白。后一组在髓鞘形成的早期阶段更为突出，并且尽管经过重复的纯化循环，仍与分离的髓磷脂膜相关。至少一种非髓磷脂亚细胞膜部分具有相同的蛋白质。培养的少突胶质细胞的总膜部分与两组GTP结合蛋白都相关。按照所述方法，没有一种著名的髓磷脂蛋白能结合GTP。
